Cell Survival Mediated by Proteins Secreted from Lactobacillus delbrueckii subsp. bulgaricus

Jasmine Sosa, University of La Verne

Abstract

Cells are constantly exposed to many traumas; while some are less harmful than others a strong stress exposure forces cells to go through apoptosis or trigger survival signals in the hopes of inducing cell recovery. Different strains of lactobacilli have shown to secrete certain factors that aid in cell recovery, one commonly known strain is Lactobacillus kefiranofaciens (L. kefir). Previous experiments with this strain determined the factor that mediated cell recovery were proteins secreted from L. kefir. In this experiment Chinese Hamster Ovary cells (CHO cells) were exposed to UV-radiation a stress that induces cell death in majority of cells. The purpose of this experiment was to determine if another lactobacillus strain, Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus), would promote cell survival in stress induced CHO cells. Comparison of the protein profiles from the two strains of lactobacilli was performed along with UV exposure to CHO cells. A dose dependent probiotic treatment was also administered followed by cell cycle analysis. Results thus far have indicated an increase in cell survival as the probiotic treatment is increased. People are constantly being exposed to UV exposure due to the effects of the sun; consequently, long periods of sun exposure can result in sun burns, sun rashes, and even skin cancer. By implementing the proteins secreted from the L. bulgaricus strain as an active ingredient to medications or creams that aid in the relief and recovery of these symptoms healing would occur in a much more rapid pace.

 
Nov 12th, 11:15 AM Nov 12th, 11:30 AM

Cell Survival Mediated by Proteins Secreted from Lactobacillus delbrueckii subsp. bulgaricus

MSE 103

Cells are constantly exposed to many traumas; while some are less harmful than others a strong stress exposure forces cells to go through apoptosis or trigger survival signals in the hopes of inducing cell recovery. Different strains of lactobacilli have shown to secrete certain factors that aid in cell recovery, one commonly known strain is Lactobacillus kefiranofaciens (L. kefir). Previous experiments with this strain determined the factor that mediated cell recovery were proteins secreted from L. kefir. In this experiment Chinese Hamster Ovary cells (CHO cells) were exposed to UV-radiation a stress that induces cell death in majority of cells. The purpose of this experiment was to determine if another lactobacillus strain, Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus), would promote cell survival in stress induced CHO cells. Comparison of the protein profiles from the two strains of lactobacilli was performed along with UV exposure to CHO cells. A dose dependent probiotic treatment was also administered followed by cell cycle analysis. Results thus far have indicated an increase in cell survival as the probiotic treatment is increased. People are constantly being exposed to UV exposure due to the effects of the sun; consequently, long periods of sun exposure can result in sun burns, sun rashes, and even skin cancer. By implementing the proteins secreted from the L. bulgaricus strain as an active ingredient to medications or creams that aid in the relief and recovery of these symptoms healing would occur in a much more rapid pace.