Presentation Title

Cytotoxicity of Electronic Cigarette Solvents: Propylene Glycol and Glycerol

Start Date

November 2016

End Date

November 2016

Location

MSE 103

Type of Presentation

Oral Talk

Abstract

The increased popularity of electronic cigarettes (ECs) has increased health concerns regarding their use. This study examines the cytotoxicity of the common EC refill fluid solvents, propylene glycol and glycerol. Aerosols of varying ratios of propylene glycol and glycerol were made using an EC (tank boxmod model) at 3 volts and 5 volts and then collected in the cell culture medium used for human pulmonary fibroblasts (hPF) and adenocarcinoma human alveolar basal epithelial cells (A549) using a smoking machine. hPF and A549 cells were then exposed to each collected aerosol and incubated for 48 hours. A cytotoxicity test (MTT assay) was used to analyze cell death and to determine whether an increase in voltage/wattage increased the cytotoxicity of aerosolized solvents. Both cell types were more sensitive to 5 volt aerosols than 3 volt aerosols. The data indicate that higher ratios of glycerol result in higher cytotoxicity. This is particularly concerning when considering refill fluids that solely use vegetable glycerol as a solvent, such as certain flavors made by Red Oak. In addition, when identical solutions were used in different tanks, cytotoxicity varied. hPF showed higher sensitivity than A549. These data indicate that variations in EC voltages, solvent ratio mixtures, and tank choice can alter produce aerosols that are cytotoxic to human lung cells.

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Nov 12th, 11:45 AM Nov 12th, 12:00 PM

Cytotoxicity of Electronic Cigarette Solvents: Propylene Glycol and Glycerol

MSE 103

The increased popularity of electronic cigarettes (ECs) has increased health concerns regarding their use. This study examines the cytotoxicity of the common EC refill fluid solvents, propylene glycol and glycerol. Aerosols of varying ratios of propylene glycol and glycerol were made using an EC (tank boxmod model) at 3 volts and 5 volts and then collected in the cell culture medium used for human pulmonary fibroblasts (hPF) and adenocarcinoma human alveolar basal epithelial cells (A549) using a smoking machine. hPF and A549 cells were then exposed to each collected aerosol and incubated for 48 hours. A cytotoxicity test (MTT assay) was used to analyze cell death and to determine whether an increase in voltage/wattage increased the cytotoxicity of aerosolized solvents. Both cell types were more sensitive to 5 volt aerosols than 3 volt aerosols. The data indicate that higher ratios of glycerol result in higher cytotoxicity. This is particularly concerning when considering refill fluids that solely use vegetable glycerol as a solvent, such as certain flavors made by Red Oak. In addition, when identical solutions were used in different tanks, cytotoxicity varied. hPF showed higher sensitivity than A549. These data indicate that variations in EC voltages, solvent ratio mixtures, and tank choice can alter produce aerosols that are cytotoxic to human lung cells.