Presentation Title

Design of Electromagnetic Field Generating Device for Biological Studies

Start Date

November 2016

End Date

November 2016

Location

HUB 379

Type of Presentation

Oral Talk

Abstract

Current stem cell research often relies on the use of growth factors to stimulate cell differentiation. However, these growth factors are not only short-lived proteins but also expensive resources. Reports have indicated that electromagnetic fields (EMFs) also have the ability to induce stem cell differentiation. In light of the lower cost and higher sustainability of EMFs compared to growth factors, we are further pursuing the use of EMFs as a potential alternative for inducing stem cell differentiation. Our project involves testing the effect of exposing bone marrow-derived mesenchymal stem cells (BMSCs) to a low to medium intensity EMF (up to 2 mT) on cell proliferation and differentiation. In order to achieve this, COMSOL Multiphysics was used to simulate six different coil configurations (Helmholtz 2 circular / square coil, Merritt 3 / 4 square coil, Lee-Whiting 4 circular coil and Alldred & Scollar 4 square coil system) for the determination of suitable coil design before the fabrication of EMF generating device. Results showed that while both the Lee-Whiting 4 circular coil system and Merritt 4 square coil system were able to achieve the same highest field intensity in the center of the system, the Lee-Whiting design required less spacing between the coils and fewer turns per coil, allowing for a more efficient use of space and materials. In conclusion, the Lee-Whiting 4 circular coil system can provide the desired EMF intensity, uniform area and coil spacing for the design of EMF generating device, which ensured the uniform exposure of an entire cell culture plate to the EMF generated by the device while the device can still fit in an incubator for the future test of cell functions.

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Nov 12th, 11:45 AM Nov 12th, 12:00 PM

Design of Electromagnetic Field Generating Device for Biological Studies

HUB 379

Current stem cell research often relies on the use of growth factors to stimulate cell differentiation. However, these growth factors are not only short-lived proteins but also expensive resources. Reports have indicated that electromagnetic fields (EMFs) also have the ability to induce stem cell differentiation. In light of the lower cost and higher sustainability of EMFs compared to growth factors, we are further pursuing the use of EMFs as a potential alternative for inducing stem cell differentiation. Our project involves testing the effect of exposing bone marrow-derived mesenchymal stem cells (BMSCs) to a low to medium intensity EMF (up to 2 mT) on cell proliferation and differentiation. In order to achieve this, COMSOL Multiphysics was used to simulate six different coil configurations (Helmholtz 2 circular / square coil, Merritt 3 / 4 square coil, Lee-Whiting 4 circular coil and Alldred & Scollar 4 square coil system) for the determination of suitable coil design before the fabrication of EMF generating device. Results showed that while both the Lee-Whiting 4 circular coil system and Merritt 4 square coil system were able to achieve the same highest field intensity in the center of the system, the Lee-Whiting design required less spacing between the coils and fewer turns per coil, allowing for a more efficient use of space and materials. In conclusion, the Lee-Whiting 4 circular coil system can provide the desired EMF intensity, uniform area and coil spacing for the design of EMF generating device, which ensured the uniform exposure of an entire cell culture plate to the EMF generated by the device while the device can still fit in an incubator for the future test of cell functions.