Presentation Title

Optimization of the Fabrication of Microfluidic Paper-Based Analytical Devices (µPADs)

Start Date

November 2016

End Date

November 2016

Location

Surge 173

Type of Presentation

Oral Talk

Abstract

Microfluidic paper-based analytical devices (µPADs) have emerged as viable multiplexable platform with the potential to transcend existing analytical techniques in resource-limited settings. µPADs are fabricated by patterning hydrophobic materials in hydrophilic paper. Paper has a number of advantages as a microfluidics platform due to its low cost and ability to wick aqueous fluids without the use of active pumping. Reproducibility in µPAD fabrication is essential in developing point-of-care (POC) diagnostic devices that utilize paper-based platforms. A critical step in fabrication involves the wax heating protocol that determines the channel dimensions. To date, no study exists that has examined variables associated with this process. Herein, we describe the effects of temperature and time of heating on line and microfluidic channel width. Analysis of the µPADs revealed that functional chips could be fabricated at temperatures between 290-420°F and time of heating between 50-135s, while non-functioning chips were obtained at temperatures between 150-290°F and time of heating between 5-45s. It was found that the wax ink spread in a consistent manner within the temperature range. This work demonstrates that continued work in the optimization of µPAD fabrication is required to develop POC devices for use in both resource-rich and limited settings.

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Nov 12th, 10:00 AM Nov 12th, 10:15 AM

Optimization of the Fabrication of Microfluidic Paper-Based Analytical Devices (µPADs)

Surge 173

Microfluidic paper-based analytical devices (µPADs) have emerged as viable multiplexable platform with the potential to transcend existing analytical techniques in resource-limited settings. µPADs are fabricated by patterning hydrophobic materials in hydrophilic paper. Paper has a number of advantages as a microfluidics platform due to its low cost and ability to wick aqueous fluids without the use of active pumping. Reproducibility in µPAD fabrication is essential in developing point-of-care (POC) diagnostic devices that utilize paper-based platforms. A critical step in fabrication involves the wax heating protocol that determines the channel dimensions. To date, no study exists that has examined variables associated with this process. Herein, we describe the effects of temperature and time of heating on line and microfluidic channel width. Analysis of the µPADs revealed that functional chips could be fabricated at temperatures between 290-420°F and time of heating between 50-135s, while non-functioning chips were obtained at temperatures between 150-290°F and time of heating between 5-45s. It was found that the wax ink spread in a consistent manner within the temperature range. This work demonstrates that continued work in the optimization of µPAD fabrication is required to develop POC devices for use in both resource-rich and limited settings.