Genetic Modification of E. coli

Sasha R. Weller, Whittier College

Abstract

The purpose of this research is to find mechanisms for gene expression under various environmental conditions. The presence of genes cheA, cheB, H858 and urvc1 in E. coli are suspected contributors to its resistance to high concentrations of alcohol and bleach. The phenotypes these genes express in the structure of the flagella, pili, fimbriae and cell wall are hypothesized to enable it to survive in these harsh conditions. We hypothesize that once expressed, these selected genes will enable E. coli to survive harsh conditions of high concentrations of bleach and ethanol. To support our hypothesis genes need to be tested for expression under normal conditions, then those expressed need to be isolated to test for resistance in varied concentrations of alcohol and bleach. First, E. coli was grown in two separate normal environmental conditions to test for expression of genes; E. coli was grown at 37⁰C in an incubator and 37⁰C in a shaker to test for motility gene expression. Our results shown via PCR consistently indicated the expression of the cheA and the suppression of cheB, H858, and uvrc1 in E. coli grown in the incubator. Our results shown via PCR indicated the expression of the cheB and the suppression of cheA, H585, and uvrc1 in E. coli grown in the shaker. These results demonstrate that this artificial selection could accurately represent what occurs in nature.

 
Nov 12th, 2:45 PM Nov 12th, 3:00 PM

Genetic Modification of E. coli

HUB 367

The purpose of this research is to find mechanisms for gene expression under various environmental conditions. The presence of genes cheA, cheB, H858 and urvc1 in E. coli are suspected contributors to its resistance to high concentrations of alcohol and bleach. The phenotypes these genes express in the structure of the flagella, pili, fimbriae and cell wall are hypothesized to enable it to survive in these harsh conditions. We hypothesize that once expressed, these selected genes will enable E. coli to survive harsh conditions of high concentrations of bleach and ethanol. To support our hypothesis genes need to be tested for expression under normal conditions, then those expressed need to be isolated to test for resistance in varied concentrations of alcohol and bleach. First, E. coli was grown in two separate normal environmental conditions to test for expression of genes; E. coli was grown at 37⁰C in an incubator and 37⁰C in a shaker to test for motility gene expression. Our results shown via PCR consistently indicated the expression of the cheA and the suppression of cheB, H858, and uvrc1 in E. coli grown in the incubator. Our results shown via PCR indicated the expression of the cheB and the suppression of cheA, H585, and uvrc1 in E. coli grown in the shaker. These results demonstrate that this artificial selection could accurately represent what occurs in nature.