The effects of Ebenaceae diospyros & Annona muricata on Mammalian Tissue Culture

Kristen A. Fregoso, Mount St. Mary's University

Abstract

Natural products have been used in medicine as a treatment for numerous diseases such as cancer. Annona muricata, the soursop, is an angiosperm that is cultivated in humid environments. For this reason, the soursop is a plant that is used in many third world countries as a primary source of treatment against cancer. Ebenaceae diospyros, the persimmon, is an angiosperm that has displayed benefits for diabetes and renal dysfunction when accompanied with other compounds like green tea leaves. Both of these species contain phytoconstituent compounds such as polyphenols and alkaloids. Phytoconstituents are compounds in plants known for their color and odor that may contribute to the beneficial effects of these plants. The objective of this study is to examine the effects of soursop and persimmon extracts on the viability of mammalian tissue culture. Our results show that soursop extracts with and without skin decreased HeLa cell viability using an MTT assay, a cytotoxicity assay, and an apoptosis kit. However, persimmon extract has shown no effect on cell viability. Further biological analysis is necessary to have a better understanding of active compounds present in the soursop that contribute to this effect.

 
Nov 12th, 10:30 AM Nov 12th, 10:45 AM

The effects of Ebenaceae diospyros & Annona muricata on Mammalian Tissue Culture

MSE 103

Natural products have been used in medicine as a treatment for numerous diseases such as cancer. Annona muricata, the soursop, is an angiosperm that is cultivated in humid environments. For this reason, the soursop is a plant that is used in many third world countries as a primary source of treatment against cancer. Ebenaceae diospyros, the persimmon, is an angiosperm that has displayed benefits for diabetes and renal dysfunction when accompanied with other compounds like green tea leaves. Both of these species contain phytoconstituent compounds such as polyphenols and alkaloids. Phytoconstituents are compounds in plants known for their color and odor that may contribute to the beneficial effects of these plants. The objective of this study is to examine the effects of soursop and persimmon extracts on the viability of mammalian tissue culture. Our results show that soursop extracts with and without skin decreased HeLa cell viability using an MTT assay, a cytotoxicity assay, and an apoptosis kit. However, persimmon extract has shown no effect on cell viability. Further biological analysis is necessary to have a better understanding of active compounds present in the soursop that contribute to this effect.