Presentation Title

Migration of Hatching Gland Cells in a Silverside Fish, the California Grunion, Leuresthes tenuis

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#64

Type of Presentation

Poster

Abstract

Previous research has identified hatching gland cells (HGCs) that contain the choriolytic enzyme(s) required to digest the outer egg shell or chorion and allow hatching in the California Grunion, Leuresthes tenuis. These cells are within the epithelium, extending from the head to the tail laterally along each embryo before, but not after, hatching. During development, HCGs appear to migrate from an anterior site of production to their final position. In an effort to investigate the mode of cell migration, we tested for the involvement of actin and myosin interactions. Embryos were incubated in a cocktail consisting of the inhibitors Jasplakinolide, Latrunculin B, and Y-27632 (JLY), at times ranging from three to seven days post fertilization. Image J software was used to analyze images of embryos to measure the maximum relative distance along the body that the HGCs had migrated. Embryos treated with JLY had cells that did not move as far, but development was also inhibited. Embryos treated with Jasplakinolide and Y-27632 alone showed reduced HGC migration, but secondary effects on muscle and circulation. Our next step is to manipulate the concentration of Latrunculin B in hopes of inhibiting cell migration without inhibiting development.

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Migration of Hatching Gland Cells in a Silverside Fish, the California Grunion, Leuresthes tenuis

HUB 302-#64

Previous research has identified hatching gland cells (HGCs) that contain the choriolytic enzyme(s) required to digest the outer egg shell or chorion and allow hatching in the California Grunion, Leuresthes tenuis. These cells are within the epithelium, extending from the head to the tail laterally along each embryo before, but not after, hatching. During development, HCGs appear to migrate from an anterior site of production to their final position. In an effort to investigate the mode of cell migration, we tested for the involvement of actin and myosin interactions. Embryos were incubated in a cocktail consisting of the inhibitors Jasplakinolide, Latrunculin B, and Y-27632 (JLY), at times ranging from three to seven days post fertilization. Image J software was used to analyze images of embryos to measure the maximum relative distance along the body that the HGCs had migrated. Embryos treated with JLY had cells that did not move as far, but development was also inhibited. Embryos treated with Jasplakinolide and Y-27632 alone showed reduced HGC migration, but secondary effects on muscle and circulation. Our next step is to manipulate the concentration of Latrunculin B in hopes of inhibiting cell migration without inhibiting development.