Presentation Title

Understanding Specific Regions of Protein P0 in the Polerovirus Genus and its Effects on Post Transcriptional Gene Silencing

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#135

Type of Presentation

Poster

Abstract

Plants have complex defensive systems to ensure their health and long term survival from environmental and pathogenic stressors. RNA silencing is a defense mechanism that plants use to eliminate viral RNAs. Our research focuses on the protein P0 from Turnip yellows virus (TuYV) and Potato leaf roll virus (PLRV), members of the Polerovirus genus. P0 functions to suppress RNA silencing mechanisms through degradation of host Argonaute proteins. While P0 overcomes RNA silencing, in the plant Nicotiana glutinosa, it triggers another defense mechanism, the hypersensitive response (HR), a type of programmed cell death that limits spread of pathogens. To study P0 functions, mutants were created by substituting six amino acids at various positions near the carboxy terminus with the sequence NAAIRS, which is known to have a minimal effect on protein secondary structure. The mutants were expressed in N. glutinosa to assay for HR or co-expressed in Nicotiana benthamiana with green fluorescent protein (GFP) to visualize RNA silencing. Essential regions for RNA silencing suppression activity and/or for elicitation of HR in the P0 protein are expected to show loss of function in the NAAIRS mutants. These experiments will provide insight into virus interactions with their host molecular defense mechanisms.

This document is currently not available here.

Share

COinS
 
Nov 12th, 4:00 PM Nov 12th, 5:00 PM

Understanding Specific Regions of Protein P0 in the Polerovirus Genus and its Effects on Post Transcriptional Gene Silencing

HUB 302-#135

Plants have complex defensive systems to ensure their health and long term survival from environmental and pathogenic stressors. RNA silencing is a defense mechanism that plants use to eliminate viral RNAs. Our research focuses on the protein P0 from Turnip yellows virus (TuYV) and Potato leaf roll virus (PLRV), members of the Polerovirus genus. P0 functions to suppress RNA silencing mechanisms through degradation of host Argonaute proteins. While P0 overcomes RNA silencing, in the plant Nicotiana glutinosa, it triggers another defense mechanism, the hypersensitive response (HR), a type of programmed cell death that limits spread of pathogens. To study P0 functions, mutants were created by substituting six amino acids at various positions near the carboxy terminus with the sequence NAAIRS, which is known to have a minimal effect on protein secondary structure. The mutants were expressed in N. glutinosa to assay for HR or co-expressed in Nicotiana benthamiana with green fluorescent protein (GFP) to visualize RNA silencing. Essential regions for RNA silencing suppression activity and/or for elicitation of HR in the P0 protein are expected to show loss of function in the NAAIRS mutants. These experiments will provide insight into virus interactions with their host molecular defense mechanisms.