Presentation Title

Epigenetic Response to an Acute Bout of Resistance Exercise in Trained Men

Start Date

November 2016

End Date

November 2016

Location

HUB 302-126

Type of Presentation

Poster

Abstract

INTRODUCTION: Epigenetics is the study of changes in gene expression of an organism in response to environmental stimuli. The human epigenetic response to resistance exercise (RE) is poorly understood. This is problematic because RE is a powerful stimulus with numerous performance and health benefits. PURPOSE: To investigate the human epigenetic response to an acute bout of heavy RE in trained males. METHODS: Resting muscle biopsies were performed on the right vastus lateralis of three highly trained males (age=26.3±1.5 y; body mass=89.1±8.4kg; ht=178.3±10.6 cm). Participants then performed three sets of 10 repetitions at 70% of their 1 repetition maximum (1RM) of a leg press and leg extension exercise (1 min rest between sets). Muscle biopsies were taken 30min and 4h post-exercise with separate incisions, ~3cm proximal to the initial biopsy location. Global DNA methylation patterns in the muscle tissue were analyzed at each time point using long interspersed nucleotide elements (LINE-1) via bisulfite pyrosequencing. Single muscle fiber type distribution was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean global DNA methylation among participants was 75.78% at rest, 73.07% at 30min post RE, and 74.36% 4h post RE. Muscle fiber type distribution was 20.00% MHC I, 11.43% MHC I/IIa, 68.57% MHC IIa, and 0.75% MHC IIa/IIx. CONCLUSION: These initial results indicate that a prescribed acute lower body RE bout was sufficient to induce a post-exercise global hypomethylation, with a subsequent global hypermethylation 4h post-exercise. The hypomethylation likely allows increased gene expression, which is necessary for long-term adaptation.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Epigenetic Response to an Acute Bout of Resistance Exercise in Trained Men

HUB 302-126

INTRODUCTION: Epigenetics is the study of changes in gene expression of an organism in response to environmental stimuli. The human epigenetic response to resistance exercise (RE) is poorly understood. This is problematic because RE is a powerful stimulus with numerous performance and health benefits. PURPOSE: To investigate the human epigenetic response to an acute bout of heavy RE in trained males. METHODS: Resting muscle biopsies were performed on the right vastus lateralis of three highly trained males (age=26.3±1.5 y; body mass=89.1±8.4kg; ht=178.3±10.6 cm). Participants then performed three sets of 10 repetitions at 70% of their 1 repetition maximum (1RM) of a leg press and leg extension exercise (1 min rest between sets). Muscle biopsies were taken 30min and 4h post-exercise with separate incisions, ~3cm proximal to the initial biopsy location. Global DNA methylation patterns in the muscle tissue were analyzed at each time point using long interspersed nucleotide elements (LINE-1) via bisulfite pyrosequencing. Single muscle fiber type distribution was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean global DNA methylation among participants was 75.78% at rest, 73.07% at 30min post RE, and 74.36% 4h post RE. Muscle fiber type distribution was 20.00% MHC I, 11.43% MHC I/IIa, 68.57% MHC IIa, and 0.75% MHC IIa/IIx. CONCLUSION: These initial results indicate that a prescribed acute lower body RE bout was sufficient to induce a post-exercise global hypomethylation, with a subsequent global hypermethylation 4h post-exercise. The hypomethylation likely allows increased gene expression, which is necessary for long-term adaptation.