Presentation Title

The Role of Kif20a Gene in Pancreatic β-cell Development

Start Date

November 2016

End Date

November 2016

Location

HUB 302-101

Type of Presentation

Poster

Abstract

Around 9.3% of the population suffers from diabetes. This condition occurs when a patient is unable to balance his or her blood glucose level, resulting in a series of severe symptoms that can become life-threatening. Diabetes falls into two categories: Type 1 and 2. Type 1 diabetes occurs when the pancreas cannot produce insulin to regulate blood glucose because of an autoimmune response targeting insulin-producing cells. Type 2 diabetes is more commonly associated with adults and lifestyle choices resulting in the loss of endocrine function. People with type 2 diabetes produce insulin, but often not enough for their body's needs. They may also struggle to use the insulin they produce effectively. In this study, knockout mice were bred to either express or not express a gene called Kif20a, which is responsible for producing a kinesin protein that is needed to facilitate proper cell division. Using methods such as genotyping, cryosectioning, immunohistochemistry, and microscope imaging, the phenotype of pancreatic tissue with Kif20a gene expression was compared to tissue that had no gene expression of Kif20a. Preliminary conclusions suggest that Kif20a could serve as a necessary component of proper cell division, but further experimentation is required to develop a thorough conclusion. By understanding the mechanisms of pancreatic development, including genes that are responsible for the proliferation and differentiation of pancreatic cells, we come closer to finding a cure to the disease.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

The Role of Kif20a Gene in Pancreatic β-cell Development

HUB 302-101

Around 9.3% of the population suffers from diabetes. This condition occurs when a patient is unable to balance his or her blood glucose level, resulting in a series of severe symptoms that can become life-threatening. Diabetes falls into two categories: Type 1 and 2. Type 1 diabetes occurs when the pancreas cannot produce insulin to regulate blood glucose because of an autoimmune response targeting insulin-producing cells. Type 2 diabetes is more commonly associated with adults and lifestyle choices resulting in the loss of endocrine function. People with type 2 diabetes produce insulin, but often not enough for their body's needs. They may also struggle to use the insulin they produce effectively. In this study, knockout mice were bred to either express or not express a gene called Kif20a, which is responsible for producing a kinesin protein that is needed to facilitate proper cell division. Using methods such as genotyping, cryosectioning, immunohistochemistry, and microscope imaging, the phenotype of pancreatic tissue with Kif20a gene expression was compared to tissue that had no gene expression of Kif20a. Preliminary conclusions suggest that Kif20a could serve as a necessary component of proper cell division, but further experimentation is required to develop a thorough conclusion. By understanding the mechanisms of pancreatic development, including genes that are responsible for the proliferation and differentiation of pancreatic cells, we come closer to finding a cure to the disease.