Presentation Title

Polyphenol Extraction from Peruvian teas and the effect of the tea fractions on HeLa cells

Start Date

November 2016

End Date

November 2016

Location

HUB 302-30

Type of Presentation

Poster

Abstract

Polyphenols are a group of naturally occurring organic compounds, consisting of many phenol rings. Anti-carcinogenic health benefits of polyphenols have been observed due to their antioxidant properties and their ability to modulate enzyme activity and cell signals. Polyphenols were collected from several Peruvian Tea mixtures made to treat different medical problems. The polyphenols were extracted using a Soxhlet setup and then were washed with chloroform to remove caffeine and pigments, then with ethyl acetate, to separate the polyphenols. The samples were concentrated, filtered and subjected to Gas Chromatography Mass Spectrometer (GC-MS) analysis. An MTT assay was performed on the samples to analyze the effects of the different individual tea extractions. It was found that the tea mixture made to alleviate nervous system cancer (Tea 1EA), decreased cell viability in HeLa cells and was consequently chosen for further analysis. This tea extract was fractioned using flash column chromatography. GC-MS analysis and a MTT assay was used on HeLa cells with all 8 fractions obtained from Tea 1EA. Our results show that fraction six, displayed a trend for lowering cell viability in HeLa cells as the concentration of the fraction increased. A compound table provided by the GC-MS showed the presence of polyphenol derivatives as well as a common PAH, Naphthalene. Currently, the effects of fractions from teas dissolved in water are being analyzed.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Polyphenol Extraction from Peruvian teas and the effect of the tea fractions on HeLa cells

HUB 302-30

Polyphenols are a group of naturally occurring organic compounds, consisting of many phenol rings. Anti-carcinogenic health benefits of polyphenols have been observed due to their antioxidant properties and their ability to modulate enzyme activity and cell signals. Polyphenols were collected from several Peruvian Tea mixtures made to treat different medical problems. The polyphenols were extracted using a Soxhlet setup and then were washed with chloroform to remove caffeine and pigments, then with ethyl acetate, to separate the polyphenols. The samples were concentrated, filtered and subjected to Gas Chromatography Mass Spectrometer (GC-MS) analysis. An MTT assay was performed on the samples to analyze the effects of the different individual tea extractions. It was found that the tea mixture made to alleviate nervous system cancer (Tea 1EA), decreased cell viability in HeLa cells and was consequently chosen for further analysis. This tea extract was fractioned using flash column chromatography. GC-MS analysis and a MTT assay was used on HeLa cells with all 8 fractions obtained from Tea 1EA. Our results show that fraction six, displayed a trend for lowering cell viability in HeLa cells as the concentration of the fraction increased. A compound table provided by the GC-MS showed the presence of polyphenol derivatives as well as a common PAH, Naphthalene. Currently, the effects of fractions from teas dissolved in water are being analyzed.