Presentation Title

Characterization of Differentially Expressed TDFs in Early Orchid Development

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#136

Type of Presentation

Poster

Abstract

Fundamental plant development, such as meristem and leaf formation, is a primary resource for working towards advancements in agricultural productivity. While model systems have been extensively researched, orchids have received little attention; the atypical development of these plants may provide unique research opportunities. Mature orchid seeds are underdeveloped compared to other angiosperms, since they lack primordial shoots or roots. Upon germination, seeds produce a tuberous, undifferentiated group of cells, the protocorm. This structure forms a meristem and leaf within a few weeks, and a root several weeks later. In this work, we identified genes that were differentially expressed during the growth of the protocorm and first leaf. Populations of mRNAs were extracted from germinating seeds of Spathoglottis plicata at four time points and cDNA amplified fragment length polymorphisms (AFLPs) were generated. The transcript-derived fragments (TDFs) which exhibited differential expression were extracted from polyacrylamide gels, subcloned and sequenced. We have identified 51 differentially expressed TDFs. One of these fragments was a GSK3/Shaggy-like kinase, which is of particular interest because Shaggy genes have been reported to be involved in brassinosteroid signaling. Several of the fragments sequenced did not produce a BLAST result, nor were they found in the published Phalaenopsis transcriptome in Orchidbase. Some of these uncharacterized genes may be unique to early development in orchids, as they did not show similarity to orchid gene transcripts that have been reported thus far.

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Characterization of Differentially Expressed TDFs in Early Orchid Development

HUB 302-#136

Fundamental plant development, such as meristem and leaf formation, is a primary resource for working towards advancements in agricultural productivity. While model systems have been extensively researched, orchids have received little attention; the atypical development of these plants may provide unique research opportunities. Mature orchid seeds are underdeveloped compared to other angiosperms, since they lack primordial shoots or roots. Upon germination, seeds produce a tuberous, undifferentiated group of cells, the protocorm. This structure forms a meristem and leaf within a few weeks, and a root several weeks later. In this work, we identified genes that were differentially expressed during the growth of the protocorm and first leaf. Populations of mRNAs were extracted from germinating seeds of Spathoglottis plicata at four time points and cDNA amplified fragment length polymorphisms (AFLPs) were generated. The transcript-derived fragments (TDFs) which exhibited differential expression were extracted from polyacrylamide gels, subcloned and sequenced. We have identified 51 differentially expressed TDFs. One of these fragments was a GSK3/Shaggy-like kinase, which is of particular interest because Shaggy genes have been reported to be involved in brassinosteroid signaling. Several of the fragments sequenced did not produce a BLAST result, nor were they found in the published Phalaenopsis transcriptome in Orchidbase. Some of these uncharacterized genes may be unique to early development in orchids, as they did not show similarity to orchid gene transcripts that have been reported thus far.