Presentation Title

Synthesis and Evaluation of Novel G-quadruplex-stabilizing Compounds

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#141

Type of Presentation

Poster

Abstract

DNA G-quadruplexes (or G-quartets) represent a new, promising target of anticancer therapies. The G-quadruplex structure, found in telomeric DNA, is composed of a stack of four guanine nucleobases formed around a metal cation, strengthened through hydrogen bonds. G-Quadruplexes have been shown to stop unrestricted cell growth in cancerous cells by inhibiting the enzyme telomerase from extending DNA length. The goal of our research is to synthesize novel molecules that stabilize the G-quadruplex structure. Through the derivatization of diaminoazobenzene and indigo based dyes, a small library of novel organic compounds was synthesized in 2-3 steps. These compounds were purified, characterized, and then tested for G-quadruplex stabilizing abilities by measuring changes in melting point curves of quadruplex-forming DNA oligomers as measured by circular dichroism. Several of the compounds increased the melting temperature of the DNA, demonstrating that these core structures possess the ability to stabilize the telomeric G-quadruplex structure.

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Synthesis and Evaluation of Novel G-quadruplex-stabilizing Compounds

HUB 302-#141

DNA G-quadruplexes (or G-quartets) represent a new, promising target of anticancer therapies. The G-quadruplex structure, found in telomeric DNA, is composed of a stack of four guanine nucleobases formed around a metal cation, strengthened through hydrogen bonds. G-Quadruplexes have been shown to stop unrestricted cell growth in cancerous cells by inhibiting the enzyme telomerase from extending DNA length. The goal of our research is to synthesize novel molecules that stabilize the G-quadruplex structure. Through the derivatization of diaminoazobenzene and indigo based dyes, a small library of novel organic compounds was synthesized in 2-3 steps. These compounds were purified, characterized, and then tested for G-quadruplex stabilizing abilities by measuring changes in melting point curves of quadruplex-forming DNA oligomers as measured by circular dichroism. Several of the compounds increased the melting temperature of the DNA, demonstrating that these core structures possess the ability to stabilize the telomeric G-quadruplex structure.