Presentation Title

Use of PCR for Detection of Mastitis-Causing Pathogens Isolated from Bovine Quarter Milk Samples

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#149

Type of Presentation

Poster

Abstract

Milk and milk products have a tremendous impact on the U.S diet in terms of achieving recommended intakes of nutrients. The dairy industry makes approximately $125 billion per year. However, this industry spends approximately $1.8 billion dollars annually in economic costs as well as veterinary costs as a result of mastitis, inflammation of the mammary gland. Mastitis can result in a decrease in milk production, among other factors. The major pathogenic agents involved in the disease are Staphylococcus aureus, Escherichia coli and Streptococci species. In this study, the potential role of pulsed electromagnetic field therapy (PEMFT) in controlling mastitis in dairy cows was investigated. A Polymerase Chain Reaction (PCR) protocol was developed in order to confirm the identity of mastitis causing pathogens isolated from 184 quarter milk (QM) samples that were collected from the udders of mastitic dairy cows treated with PEMFT. The PCR protocol utilizes S. aureus, E. coli, and three Streptococcus sp. pathogen-specific primers that amplify a specific segment of DNA in these bacterial strains. The multiplex PCR protocol currently includes five primer sets. Once DNA amplification is achieved, gel electrophoresis allows for comparison of the DNA banding patterns of quality control (QC) strains with isolates from QM samples. A total of 56 of the QM samples were evaluated, and 32 (57%) tested positive for E. coli and 1 (2%) for Staphylococcus. The effect of PEMFT on mastitis will be assessed after all the samples have been tested. It is hypothesized PEMFT has the potential of becoming an effective, non-invasive and antibiotic–free treatment capable of reducing mastitis pathogens.

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Use of PCR for Detection of Mastitis-Causing Pathogens Isolated from Bovine Quarter Milk Samples

HUB 302-#149

Milk and milk products have a tremendous impact on the U.S diet in terms of achieving recommended intakes of nutrients. The dairy industry makes approximately $125 billion per year. However, this industry spends approximately $1.8 billion dollars annually in economic costs as well as veterinary costs as a result of mastitis, inflammation of the mammary gland. Mastitis can result in a decrease in milk production, among other factors. The major pathogenic agents involved in the disease are Staphylococcus aureus, Escherichia coli and Streptococci species. In this study, the potential role of pulsed electromagnetic field therapy (PEMFT) in controlling mastitis in dairy cows was investigated. A Polymerase Chain Reaction (PCR) protocol was developed in order to confirm the identity of mastitis causing pathogens isolated from 184 quarter milk (QM) samples that were collected from the udders of mastitic dairy cows treated with PEMFT. The PCR protocol utilizes S. aureus, E. coli, and three Streptococcus sp. pathogen-specific primers that amplify a specific segment of DNA in these bacterial strains. The multiplex PCR protocol currently includes five primer sets. Once DNA amplification is achieved, gel electrophoresis allows for comparison of the DNA banding patterns of quality control (QC) strains with isolates from QM samples. A total of 56 of the QM samples were evaluated, and 32 (57%) tested positive for E. coli and 1 (2%) for Staphylococcus. The effect of PEMFT on mastitis will be assessed after all the samples have been tested. It is hypothesized PEMFT has the potential of becoming an effective, non-invasive and antibiotic–free treatment capable of reducing mastitis pathogens.