Presentation Title

Extraction and Quantification of Total Carotenoids Using Green Solvents

Start Date

November 2016

End Date

November 2016

Location

HUB 302-163

Type of Presentation

Poster

Abstract

Carotenoids are a yellow to red pigment found primarily in plants which provide various health benefits such as boosting immune system, vision and other health benefits. There are many different carotenoids found in nature and few are vitamin A precursors and antioxidants. Over the years many experiments have been conducted on extracting carotenoids from fruits and vegetables using environmentally unsafe organic solvents such as hexane and acetone. The purpose of this experiment was to determine whether or not green solvents can be a successful substitute for conventional organic solvents within classroom applications. Coconut oil, sunflower oil, safflower oil, and grapeseed oil were used to extract carotenoids using solid-liquid and liquid-liquid extraction. The absorbances of the resulting extracts were analyzed using a spectrophotometer. An established calibration curve was used to calculate the concentration of carotenoids within the samples. The concentrations were then used to calculate the mass of the carotenoids within the samples. The concentrations were then used to calculate the mass of carotenoids in micrograms per gram of sample (μg/g). The results indicated that coconut oil was the most efficient solvent with a mean of 207 μg/g (∓8.24). The lowest extraction value was found to belong to grapeseed oil with a mean of 143 μg/g (∓18). It was concluded that of all the green solvents used, the coconut oil provided an efficient and sustainable method for the extraction of carotenoids; the similarities in polarity between the oil and non polar carotenoids, coupled with the oil’s transparency, it was a perfect platform to extract the carotenoids in red yams.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Extraction and Quantification of Total Carotenoids Using Green Solvents

HUB 302-163

Carotenoids are a yellow to red pigment found primarily in plants which provide various health benefits such as boosting immune system, vision and other health benefits. There are many different carotenoids found in nature and few are vitamin A precursors and antioxidants. Over the years many experiments have been conducted on extracting carotenoids from fruits and vegetables using environmentally unsafe organic solvents such as hexane and acetone. The purpose of this experiment was to determine whether or not green solvents can be a successful substitute for conventional organic solvents within classroom applications. Coconut oil, sunflower oil, safflower oil, and grapeseed oil were used to extract carotenoids using solid-liquid and liquid-liquid extraction. The absorbances of the resulting extracts were analyzed using a spectrophotometer. An established calibration curve was used to calculate the concentration of carotenoids within the samples. The concentrations were then used to calculate the mass of the carotenoids within the samples. The concentrations were then used to calculate the mass of carotenoids in micrograms per gram of sample (μg/g). The results indicated that coconut oil was the most efficient solvent with a mean of 207 μg/g (∓8.24). The lowest extraction value was found to belong to grapeseed oil with a mean of 143 μg/g (∓18). It was concluded that of all the green solvents used, the coconut oil provided an efficient and sustainable method for the extraction of carotenoids; the similarities in polarity between the oil and non polar carotenoids, coupled with the oil’s transparency, it was a perfect platform to extract the carotenoids in red yams.