Presentation Title

Hop Topic: The Effects of Genistein, a Phytoestrogen in Beer Brewery Wastewater, on the Reproductive Physiology of Zebrafish (Danio rerio)

Start Date

November 2016

End Date

November 2016

Location

HUB 302-102

Type of Presentation

Poster

Abstract

Beer brewing is a growing industry and is responsible for an estimated $6.8 billion in annual revenue in California. However, for one liter of product, up to 10 liters of wastewater, the excess water and plant matter generated in the brewing process are produced. The plant matter in brewery wastewater contains compounds (e.g. phytoestrogens) which have the potential to mimic estrogen hormones produced by the vertebrate endocrine system. Thus, phytoestrogens may evoke a similar response to estrogen by binding to the same hormone receptors. Our hypothesis is that the phytoestrogen genistein, isolated from brewery wastewater, may affect the reproductive physiology of young fish in a dose-dependent manner. Two-week-old zebrafish (Danio rerio; n=358) were exposed in triplicate to genistein (5, 50, and 500 μg/L), a positive control (0.1 μg/L estradiol 17-β), and a negative control (ethanol) for eight weeks. After the exposure period, standard length (mm) and body mass (mg) were measured. Fish exposed to genistein were significantly greater in standard length (p<0.0001) and weight (p<0.0001) compared to positive control fish. Currently, reproductive tissues (i.e., ovary and testis) are being examined histologically to determine differences in gonadal development. Genistein is an important phytoestrogen to study as it may affect developmental reproductive physiology of aquatic organisms and vertebrates. Increased awareness of phytoestrogens and the brewing industry will be important as microbreweries grow in popularity and in protection of wildlife.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Hop Topic: The Effects of Genistein, a Phytoestrogen in Beer Brewery Wastewater, on the Reproductive Physiology of Zebrafish (Danio rerio)

HUB 302-102

Beer brewing is a growing industry and is responsible for an estimated $6.8 billion in annual revenue in California. However, for one liter of product, up to 10 liters of wastewater, the excess water and plant matter generated in the brewing process are produced. The plant matter in brewery wastewater contains compounds (e.g. phytoestrogens) which have the potential to mimic estrogen hormones produced by the vertebrate endocrine system. Thus, phytoestrogens may evoke a similar response to estrogen by binding to the same hormone receptors. Our hypothesis is that the phytoestrogen genistein, isolated from brewery wastewater, may affect the reproductive physiology of young fish in a dose-dependent manner. Two-week-old zebrafish (Danio rerio; n=358) were exposed in triplicate to genistein (5, 50, and 500 μg/L), a positive control (0.1 μg/L estradiol 17-β), and a negative control (ethanol) for eight weeks. After the exposure period, standard length (mm) and body mass (mg) were measured. Fish exposed to genistein were significantly greater in standard length (p<0.0001) and weight (p<0.0001) compared to positive control fish. Currently, reproductive tissues (i.e., ovary and testis) are being examined histologically to determine differences in gonadal development. Genistein is an important phytoestrogen to study as it may affect developmental reproductive physiology of aquatic organisms and vertebrates. Increased awareness of phytoestrogens and the brewing industry will be important as microbreweries grow in popularity and in protection of wildlife.