Presentation Title

AVG suppresses glucose accumulation in the endosperm of developing kernels cultured under death-promoting sucrose levels

Start Date

November 2016

End Date

November 2016

Location

HUB 302-55

Type of Presentation

Poster

Abstract

Grain fill during maize kernel development creates a dynamic situation with fluctuating levels of sucrose, glucose and starch. Previous work in our lab demonstrated that starchy kernels exhibit enhanced programmed cell death (PCD) when cultured under high sucrose conditions. In vivo, as sucrose enters a kernel during grain fill, an invertase cleaves the disaccharide. These simple sugars may form trehalose, a common signaling sugar, which contributes to changes in gene expression, leading to PCD or other developmental changes. To further evaluate our culture system, kernels were collected before PCD had begun, at 12, 14, and 16 days after pollination (DAP), and cultured overnight in sucrose with or without AVG, since researchers have demonstrated that ethylene plays a role in endosperm PCD. Median sections were stained for viability or frozen for later glucose analysis using a glucose oxidase assay kit. Both the 14 and 16 DAP kernels in the water control showed some death, but sucrose-treated kernels at all three time points exhibited considerably more death. Kernels cultured with sucrose plus AVG exhibited no death. Glucose assay results were somewhat unexpected. The 20% treatment exhibited glucose accumulation comparable to the control kernels and the presence of AVG glucose accumulation decreased. These finding suggest that ethylene may play a role in promoting invertase activity and regulating the quantity of glucose accumulation in the endosperm.

This document is currently not available here.

Share

COinS
 
Nov 12th, 1:00 PM Nov 12th, 2:00 PM

AVG suppresses glucose accumulation in the endosperm of developing kernels cultured under death-promoting sucrose levels

HUB 302-55

Grain fill during maize kernel development creates a dynamic situation with fluctuating levels of sucrose, glucose and starch. Previous work in our lab demonstrated that starchy kernels exhibit enhanced programmed cell death (PCD) when cultured under high sucrose conditions. In vivo, as sucrose enters a kernel during grain fill, an invertase cleaves the disaccharide. These simple sugars may form trehalose, a common signaling sugar, which contributes to changes in gene expression, leading to PCD or other developmental changes. To further evaluate our culture system, kernels were collected before PCD had begun, at 12, 14, and 16 days after pollination (DAP), and cultured overnight in sucrose with or without AVG, since researchers have demonstrated that ethylene plays a role in endosperm PCD. Median sections were stained for viability or frozen for later glucose analysis using a glucose oxidase assay kit. Both the 14 and 16 DAP kernels in the water control showed some death, but sucrose-treated kernels at all three time points exhibited considerably more death. Kernels cultured with sucrose plus AVG exhibited no death. Glucose assay results were somewhat unexpected. The 20% treatment exhibited glucose accumulation comparable to the control kernels and the presence of AVG glucose accumulation decreased. These finding suggest that ethylene may play a role in promoting invertase activity and regulating the quantity of glucose accumulation in the endosperm.