Presentation Title

The toxic effects of 2- and 6-hydroxychrysene on the development of Danio rerio embryos and larvae

Start Date

November 2016

End Date

November 2016

Location

HUB 302-188

Type of Presentation

Poster

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are components of crude oil that are known to be toxic to aquatic organisms. An example of a PAH found in oil is chrysene. Chrysene undergoes photochemical reactions resulting in hydroxylated PAHs such as 2- and 6-hydroxychrysene. Using the zebrafish model, embryos were exposed to 2- and 6-hydroxychrysene at 2 hours post fertilization (hpf) for a 74 hour period. The concentrations ranged from 0.5-10µM. The fish were observed at 76 hpf for survival and developmental defects. Although the most classical deformities, caused by PAHs are cardiac defects, other abnormalities were also observed. They included curved-body axis, bent tails, eye, and circulation defects. An average of 94.4%± 4.4, 90.3% ± 3.9, and 85.9% ± 5.0 of fish were observed with circulation defects for those treated with 3, 5, and 10 µM 2-hydroxychrysene, respectively. Eye defects were also observed with average values of 82.5% ± 6.8 (3µM), 96.0% ± 4.0 (5µM), and 97.4% ± 1.9 (10µM). Cardiac effects were the highest at 10 µM 2 hydroxychrysene (76% ± 8) and 5 µM 6 hydroxchyrsene (80% ± 9). The results reveal that chrysene photoproducts produced defects during zebrafish development, and that further study identifying mechanisms involved in the toxicity of photoproducts is needed to better assess the potential risks of oil spills on fish.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

The toxic effects of 2- and 6-hydroxychrysene on the development of Danio rerio embryos and larvae

HUB 302-188

Polycyclic aromatic hydrocarbons (PAHs) are components of crude oil that are known to be toxic to aquatic organisms. An example of a PAH found in oil is chrysene. Chrysene undergoes photochemical reactions resulting in hydroxylated PAHs such as 2- and 6-hydroxychrysene. Using the zebrafish model, embryos were exposed to 2- and 6-hydroxychrysene at 2 hours post fertilization (hpf) for a 74 hour period. The concentrations ranged from 0.5-10µM. The fish were observed at 76 hpf for survival and developmental defects. Although the most classical deformities, caused by PAHs are cardiac defects, other abnormalities were also observed. They included curved-body axis, bent tails, eye, and circulation defects. An average of 94.4%± 4.4, 90.3% ± 3.9, and 85.9% ± 5.0 of fish were observed with circulation defects for those treated with 3, 5, and 10 µM 2-hydroxychrysene, respectively. Eye defects were also observed with average values of 82.5% ± 6.8 (3µM), 96.0% ± 4.0 (5µM), and 97.4% ± 1.9 (10µM). Cardiac effects were the highest at 10 µM 2 hydroxychrysene (76% ± 8) and 5 µM 6 hydroxchyrsene (80% ± 9). The results reveal that chrysene photoproducts produced defects during zebrafish development, and that further study identifying mechanisms involved in the toxicity of photoproducts is needed to better assess the potential risks of oil spills on fish.