Presentation Title

The Role of the Polycomb Repressive Complex Proteins on the Development of Prostate, Breast, and Colon Cancers

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#49

Type of Presentation

Poster

Abstract

The Polycomb Group proteins play an important role in cellular differentiation during development by repressing transcription. These proteins make up two Polycomb Repressive Complexes (PRC 1 and PRC 2). PRC 1 contains two homologous proteins, BMI-1 and Mel-18 that have been found to have a relationship in the development of prostate cancer. In recent studies, an inversely proportional relationship between BMI-1 and Mel-18 has been identified. It was shown that over expression of BMI-1 is present in malignant tumor cells, whereas Mel-18 is not as expressed. In the normal and benign tumors, there were higher levels of Mel-18 being expressed and lower levels of BMI-1. The purpose of our research is to analyze the effect that BMI-1 and Mel-18 have on the viability of DU-145, medium malignant prostate cancer cell line, and HCT116, malignant colon cancer cell line. The BMI-1 and Mel-18 genes were cloned into a pcDNA3.1 vector and transfected into DU-145 and HCT116 cells. We observed the cell viability of the transfected DU-145 and HCT116 cells by performing a MTT (methylthiasol tetrazolium) Assay over different time intervals of 24, 48, and 72 hours incubation. We expected to see that BMI-1 transfected cells have a higher viability and Mel-18 transfected cells to have a lower viability. A western blot, using the BMI-1 and Mel-18 antibodies, was performed to check if the transfection was successful. Our results displayed that there was an increase in viability when transfected with BMI and thus must have a correlated role with cancer progression.

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The Role of the Polycomb Repressive Complex Proteins on the Development of Prostate, Breast, and Colon Cancers

HUB 302-#49

The Polycomb Group proteins play an important role in cellular differentiation during development by repressing transcription. These proteins make up two Polycomb Repressive Complexes (PRC 1 and PRC 2). PRC 1 contains two homologous proteins, BMI-1 and Mel-18 that have been found to have a relationship in the development of prostate cancer. In recent studies, an inversely proportional relationship between BMI-1 and Mel-18 has been identified. It was shown that over expression of BMI-1 is present in malignant tumor cells, whereas Mel-18 is not as expressed. In the normal and benign tumors, there were higher levels of Mel-18 being expressed and lower levels of BMI-1. The purpose of our research is to analyze the effect that BMI-1 and Mel-18 have on the viability of DU-145, medium malignant prostate cancer cell line, and HCT116, malignant colon cancer cell line. The BMI-1 and Mel-18 genes were cloned into a pcDNA3.1 vector and transfected into DU-145 and HCT116 cells. We observed the cell viability of the transfected DU-145 and HCT116 cells by performing a MTT (methylthiasol tetrazolium) Assay over different time intervals of 24, 48, and 72 hours incubation. We expected to see that BMI-1 transfected cells have a higher viability and Mel-18 transfected cells to have a lower viability. A western blot, using the BMI-1 and Mel-18 antibodies, was performed to check if the transfection was successful. Our results displayed that there was an increase in viability when transfected with BMI and thus must have a correlated role with cancer progression.