Presentation Title

Investigation of Vertical Transmission Across the Lactobacillus Genus

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#51

Type of Presentation

Poster

Abstract

Research has shown that Lactobacillus bacteria are important to their host as part of the microbial community living on and inside them, or their microbiome. Lactobacilli found in humans are linked to fitness benefits such as strengthening the immune system, treatment in respiratory infections, and assistance in digestive health. In our study, we looked at Lactobacillus transmission modes such as vertical transmission, or mother to child transmission, and horizontal transmission, or transmission through the environment or other non-familial individuals. By studying the phylogeny of the microbiota in relation to transmission modes we can potentially learn how transmission modes evolved. We expect vertical transmission in Lactobacillus to appear multiple times across many small clades of Lactobacillus while the rest occur as horizontally transmitted bacteria. To detect trends in transmission mode evolution we constructed a phylogenetic tree of 71 coding DNA sequences from 214 Lactobacillus strains. In contrast to the original phylogenetic tree we constructed a codon aware phylogeny. Additionally, we overlaid known transmission modes of Lactobacilli obtained from papers that sequenced the genomes of the bacteria. Most of the Lactobacilli were vertically transmitted in one clade in the phylogenetic tree. This suggests that they have a common ancestor that was vertically transmitted. The remaining bacteria in the clade may have lost the capability for vertical transmission and instead used horizontal transmission. Current data available on transmission modes of Lactobacilli is limited and a more comprehensive study in this area would reveal more trends in transmission modes.

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Nov 12th, 4:00 PM Nov 12th, 5:00 PM

Investigation of Vertical Transmission Across the Lactobacillus Genus

HUB 302-#51

Research has shown that Lactobacillus bacteria are important to their host as part of the microbial community living on and inside them, or their microbiome. Lactobacilli found in humans are linked to fitness benefits such as strengthening the immune system, treatment in respiratory infections, and assistance in digestive health. In our study, we looked at Lactobacillus transmission modes such as vertical transmission, or mother to child transmission, and horizontal transmission, or transmission through the environment or other non-familial individuals. By studying the phylogeny of the microbiota in relation to transmission modes we can potentially learn how transmission modes evolved. We expect vertical transmission in Lactobacillus to appear multiple times across many small clades of Lactobacillus while the rest occur as horizontally transmitted bacteria. To detect trends in transmission mode evolution we constructed a phylogenetic tree of 71 coding DNA sequences from 214 Lactobacillus strains. In contrast to the original phylogenetic tree we constructed a codon aware phylogeny. Additionally, we overlaid known transmission modes of Lactobacilli obtained from papers that sequenced the genomes of the bacteria. Most of the Lactobacilli were vertically transmitted in one clade in the phylogenetic tree. This suggests that they have a common ancestor that was vertically transmitted. The remaining bacteria in the clade may have lost the capability for vertical transmission and instead used horizontal transmission. Current data available on transmission modes of Lactobacilli is limited and a more comprehensive study in this area would reveal more trends in transmission modes.