Presentation Title

Role of Integrins in Canine Cancer Cells

Start Date

November 2016

End Date

November 2016

Location

HUB 302-68

Type of Presentation

Poster

Abstract

Integrins are transmembrane proteins present of the surface of cells Integrins aid the cell in many tasks, such as adhesion, proliferation, migration, growth, cell-to-cell contact, and connecting the cell to the extracellular matrix. The purpose of this study is to determine which integrins are present on the surface of a line of canine cancer cells, named D17. Cells were handled in a biohazard, sterile hood and fed with Eagle MEM media. Several assays were conducted to test for presence or function of integrins on D17s, including an integrin inhibition assay, scratch wound assay, and the cells were run through the flow cytometer to determine the presence of integrins on the surface of the cells. The integrin inhibition assay tests the adhesion and attachment of the cells via integrins. The scratch wound assay is a test of migration potential and data from flow cytometry is an indicator of which integrins are present on the surface of the cells and which are not. So far we know that a6 and b1 integrins are present on the surface of D17 cells. We are beginning to understand the relationship between integrins and adhesion, migration or attachment of the cells. By learning more about D17 cells, they can be used as a model to better understand other cancer cell lines.

This document is currently not available here.

Share

COinS
 
Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Role of Integrins in Canine Cancer Cells

HUB 302-68

Integrins are transmembrane proteins present of the surface of cells Integrins aid the cell in many tasks, such as adhesion, proliferation, migration, growth, cell-to-cell contact, and connecting the cell to the extracellular matrix. The purpose of this study is to determine which integrins are present on the surface of a line of canine cancer cells, named D17. Cells were handled in a biohazard, sterile hood and fed with Eagle MEM media. Several assays were conducted to test for presence or function of integrins on D17s, including an integrin inhibition assay, scratch wound assay, and the cells were run through the flow cytometer to determine the presence of integrins on the surface of the cells. The integrin inhibition assay tests the adhesion and attachment of the cells via integrins. The scratch wound assay is a test of migration potential and data from flow cytometry is an indicator of which integrins are present on the surface of the cells and which are not. So far we know that a6 and b1 integrins are present on the surface of D17 cells. We are beginning to understand the relationship between integrins and adhesion, migration or attachment of the cells. By learning more about D17 cells, they can be used as a model to better understand other cancer cell lines.