Presentation Title

Nitrite Reducing Bacteria Isolated from Groundwater and Sediment from the Oak Ridge Field Research Center

Start Date

November 2016

End Date

November 2016

Location

HUB 302-#106

Type of Presentation

Poster

Abstract

Microbes’ and microbial communities’ impact on their ecosystems are not well understood. We work with microbes directly obtained from the environment to try and better understand them. We studied the effects of nitrite on the system because it is the first intermediate in the pathway for nitrate reduction and is also known to be toxic to bacteria. These results have implications for future research on how microbes interact under other conditions or in different environments. We work to isolate novel strains of bacteria from their environments and study their physiology. In this research, nitrite respiring bacteria were isolated from sediment through enrichment for carbon utilization under nitrite reducing conditions. Additionally, three naturally co-existing strains of Pseudomonas fluorescens that were previously isolated from groundwater were cultivated in mono-cultures and co-cultures under denitrifying conditions to study their physiology and explore their tolerance and sensitivity to nitrite. These cultures were exposed to different concentrations of nitrite in 48 well plates and their growth was monitored by optical density(600nm).

This document is currently not available here.

Share

COinS
 
Nov 12th, 4:00 PM Nov 12th, 5:00 PM

Nitrite Reducing Bacteria Isolated from Groundwater and Sediment from the Oak Ridge Field Research Center

HUB 302-#106

Microbes’ and microbial communities’ impact on their ecosystems are not well understood. We work with microbes directly obtained from the environment to try and better understand them. We studied the effects of nitrite on the system because it is the first intermediate in the pathway for nitrate reduction and is also known to be toxic to bacteria. These results have implications for future research on how microbes interact under other conditions or in different environments. We work to isolate novel strains of bacteria from their environments and study their physiology. In this research, nitrite respiring bacteria were isolated from sediment through enrichment for carbon utilization under nitrite reducing conditions. Additionally, three naturally co-existing strains of Pseudomonas fluorescens that were previously isolated from groundwater were cultivated in mono-cultures and co-cultures under denitrifying conditions to study their physiology and explore their tolerance and sensitivity to nitrite. These cultures were exposed to different concentrations of nitrite in 48 well plates and their growth was monitored by optical density(600nm).