Presentation Title

Investigation of the Metabolic Gene Deregulation in Docetaxel-resistant MCF-7 Breast Carcinoma Cells

Start Date

November 2016

End Date

November 2016

Location

HUB 302-148

Type of Presentation

Poster

Abstract

Breast cancer is among the leading causes of mortality for women in the United States. Despite the effectiveness of chemotherapy in eradicating tumor cells, some cells eventually become resistant leading to more aggressive tumor recurrence. The precise molecular basis of cancer cell resistance remains unclear. Epithelial-to-mesenchymal transition (EMT) – the mechanism by which cells lose polarity and become more migratory and invasive – and cell cycle deregulation are known to play a role in cancer progression. The current studies examine the effect of docetaxel induced chemo-resistance in MCF-7 human breast cancer cells on epithelial-to-mesenchymal transition (EMT) and cell cycle progression. The data suggest that (1) docetaxel-resistant MCF-7 cells exhibit morphological changes, (2) undergo EMT and (3) decrease cell cycle progression compared to the untreated parental MCF-7 cells. These findings suggest that EMT and decreased cell cycle progression play a role in chemo resistant breast cancer cells. Future studies will focus on: (1) the motility differences between the parental and the resistant cells and (2) differential gene expression of metabolic genes by qPCR, and EMT biomarker expression between Parental MCF-7 and docetaxel resistant MCF-7 cells, to better understand the molecular basis of chemo-resistance.

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Nov 12th, 1:00 PM Nov 12th, 2:00 PM

Investigation of the Metabolic Gene Deregulation in Docetaxel-resistant MCF-7 Breast Carcinoma Cells

HUB 302-148

Breast cancer is among the leading causes of mortality for women in the United States. Despite the effectiveness of chemotherapy in eradicating tumor cells, some cells eventually become resistant leading to more aggressive tumor recurrence. The precise molecular basis of cancer cell resistance remains unclear. Epithelial-to-mesenchymal transition (EMT) – the mechanism by which cells lose polarity and become more migratory and invasive – and cell cycle deregulation are known to play a role in cancer progression. The current studies examine the effect of docetaxel induced chemo-resistance in MCF-7 human breast cancer cells on epithelial-to-mesenchymal transition (EMT) and cell cycle progression. The data suggest that (1) docetaxel-resistant MCF-7 cells exhibit morphological changes, (2) undergo EMT and (3) decrease cell cycle progression compared to the untreated parental MCF-7 cells. These findings suggest that EMT and decreased cell cycle progression play a role in chemo resistant breast cancer cells. Future studies will focus on: (1) the motility differences between the parental and the resistant cells and (2) differential gene expression of metabolic genes by qPCR, and EMT biomarker expression between Parental MCF-7 and docetaxel resistant MCF-7 cells, to better understand the molecular basis of chemo-resistance.