Presentation Title

Ethyl Acetate Extraction of Morinda citrofolia and Its Effect on the Viability and Cytotoxicity of HeLa Cells

Faculty Mentor

Luiza A. Nogaj

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

HARBESON 42

Session

POSTER 2

Type of Presentation

Poster

Subject Area

health_nutrition_clinical_science

Abstract

Cervical and breast cancer remain two of the most prevalent types of cancer in the world. Although Western industrialized nations rely on a contemporary approach to modern medicine, many developing countries, such as Peru, rely on traditional medicine and alternative natural remedies. The focus of this study was to determine the extent to which natural remedies and nutrition, contribute to the prevention and incidence of cancer. Here, we focus on the use of noni fruit as a natural remedy for illness. Noni or Morinda citrofolia is a small tree that can be found in moderately wet or tropical environments throughout the Pacific, Southeast Asia, Australia, and South America, such as Peru. Traditionally used in Polynesian culture for treating kidney problems, diabetes, and even cancer, noni is now marketed in stores globally for its health benefits. First, survey data was collected in Cusco, Peru regarding which fruits are used as natural remedies for illness. The survey results showed noni as one of the most commonly used fruits. Molecular techniques such as MTT and LDH Cytotoxicity assays were used to test the effect this fruit and its leaves had on HeLa cells. Samples of noni powder and leaves were extracted with ethyl acetate and used in MTT and cytotoxicity assays. Preliminary results in mammalian cells showed a significant decrease in cell viability upon addition of the ethyl acetate noni extracts. The study was expanded by testing samples of green and yellow noni fruit, pulp, and skin. Each of the extracts resulted in a significant decrease in HeLa cell viability. Further analysis is necessary to examine the molecular mechanism of noni extracts on cell viability.

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Ethyl Acetate Extraction of Morinda citrofolia and Its Effect on the Viability and Cytotoxicity of HeLa Cells

HARBESON 42

Cervical and breast cancer remain two of the most prevalent types of cancer in the world. Although Western industrialized nations rely on a contemporary approach to modern medicine, many developing countries, such as Peru, rely on traditional medicine and alternative natural remedies. The focus of this study was to determine the extent to which natural remedies and nutrition, contribute to the prevention and incidence of cancer. Here, we focus on the use of noni fruit as a natural remedy for illness. Noni or Morinda citrofolia is a small tree that can be found in moderately wet or tropical environments throughout the Pacific, Southeast Asia, Australia, and South America, such as Peru. Traditionally used in Polynesian culture for treating kidney problems, diabetes, and even cancer, noni is now marketed in stores globally for its health benefits. First, survey data was collected in Cusco, Peru regarding which fruits are used as natural remedies for illness. The survey results showed noni as one of the most commonly used fruits. Molecular techniques such as MTT and LDH Cytotoxicity assays were used to test the effect this fruit and its leaves had on HeLa cells. Samples of noni powder and leaves were extracted with ethyl acetate and used in MTT and cytotoxicity assays. Preliminary results in mammalian cells showed a significant decrease in cell viability upon addition of the ethyl acetate noni extracts. The study was expanded by testing samples of green and yellow noni fruit, pulp, and skin. Each of the extracts resulted in a significant decrease in HeLa cell viability. Further analysis is necessary to examine the molecular mechanism of noni extracts on cell viability.