Presentation Title

The Effects of Cafestol on Cancer

Faculty Mentor

Sylvia Vetrone

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 87

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Coffee is consumed daily by many people around the world for its caffeine effects but can also be a major anticarcinogen. Cafestol is found in high amounts in unfiltered coffees as it is found in the coffee bean itself. Cafestol has been presented to have antioxidant, anti-inflammatory, anti-mutagenic and anti-carcinogenic effects on various leukemia and kidney cell lines. Due to Cafestol’s effectiveness on both adherent and non-adherent cell lines, we hypothesize that Cafestol would work just as well on adherent cell lines, MCF7 and LNCAP, along with non-adherent cell lines, RMPI 8226 and CML. The predicted effect Cafestol would have on all cell lines is to decrease cell viability and increase apoptosis. In this experiment a dose response Cell Titer96 Assay and Apotox Glo Triplex Assay were conducted at concentrations of 0 uM, 30 uM, 60 uM, 90 uM, 120 uM and 150 uM and time of 48 hours and 72 hours in order to test cell viability, apoptotic patterns, and cytotoxicity. According to the study, Cafestol significantly decreased the number of viable cells at 60 uM regardless of adherence or cell line. Additional assays are still being conducted to test the pathways of cellular death in order to get a better understanding at which specific location Cafestol is targeting cancer cells. In conclusion, cafestol reduced the amount of living cancer cells best at 60 mM indiscriminate of the type of cell lines conducted in the study.

This document is currently not available here.

Share

COinS
 
Nov 17th, 8:30 AM Nov 17th, 10:30 AM

The Effects of Cafestol on Cancer

CREVELING 87

Coffee is consumed daily by many people around the world for its caffeine effects but can also be a major anticarcinogen. Cafestol is found in high amounts in unfiltered coffees as it is found in the coffee bean itself. Cafestol has been presented to have antioxidant, anti-inflammatory, anti-mutagenic and anti-carcinogenic effects on various leukemia and kidney cell lines. Due to Cafestol’s effectiveness on both adherent and non-adherent cell lines, we hypothesize that Cafestol would work just as well on adherent cell lines, MCF7 and LNCAP, along with non-adherent cell lines, RMPI 8226 and CML. The predicted effect Cafestol would have on all cell lines is to decrease cell viability and increase apoptosis. In this experiment a dose response Cell Titer96 Assay and Apotox Glo Triplex Assay were conducted at concentrations of 0 uM, 30 uM, 60 uM, 90 uM, 120 uM and 150 uM and time of 48 hours and 72 hours in order to test cell viability, apoptotic patterns, and cytotoxicity. According to the study, Cafestol significantly decreased the number of viable cells at 60 uM regardless of adherence or cell line. Additional assays are still being conducted to test the pathways of cellular death in order to get a better understanding at which specific location Cafestol is targeting cancer cells. In conclusion, cafestol reduced the amount of living cancer cells best at 60 mM indiscriminate of the type of cell lines conducted in the study.