Presentation Title

Functional Analysis of Mitogen-Activated Protein Kinase 9 (MPK9) in Arabidopsis thaliana.

Faculty Mentor

Fabien Jammes

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 94

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Mitogen-activated protein kinases (MAPKs) are enzymes involved in signaling transduction that can be found in practically all eukaryotes. In plants, MAPKs are important in numerous processes, including plant development and adaptation to the environment. MPK9 and MPK12 in particular have been identified in Arabidopsis thaliana as positive regulators of the reactive oxygen species-mediated abscisic acid (ABA) hormone signaling pathway through which plants adapt to drought. This ABA signaling pathway plays a major role in plant adaptation to water stress by promoting stomata closure, which reduces transpiration by the plant and thus diminishes water loss. A recent yeast-two-hybrid assay on MPK9 identified several potential protein partners with which it may interact. To confirm these protein interactions, coding sequences of the protein partners were cloned into expression vectors through which they can be purified and used for in vitro kinase assays or co-immunoprecipitation. Additionally, the MPK9 coding sequence and its promoter were cloned into various plant vectors and used to make transgenic plants allowing for in vivo visualization of the subcellular localization of the protein and the expression pattern of the gene. These studies on MPK9 and its partners will work to shed light on its function in the ABA signaling pathway and in the plant as a whole.

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Nov 17th, 8:30 AM Nov 17th, 10:30 AM

Functional Analysis of Mitogen-Activated Protein Kinase 9 (MPK9) in Arabidopsis thaliana.

CREVELING 94

Mitogen-activated protein kinases (MAPKs) are enzymes involved in signaling transduction that can be found in practically all eukaryotes. In plants, MAPKs are important in numerous processes, including plant development and adaptation to the environment. MPK9 and MPK12 in particular have been identified in Arabidopsis thaliana as positive regulators of the reactive oxygen species-mediated abscisic acid (ABA) hormone signaling pathway through which plants adapt to drought. This ABA signaling pathway plays a major role in plant adaptation to water stress by promoting stomata closure, which reduces transpiration by the plant and thus diminishes water loss. A recent yeast-two-hybrid assay on MPK9 identified several potential protein partners with which it may interact. To confirm these protein interactions, coding sequences of the protein partners were cloned into expression vectors through which they can be purified and used for in vitro kinase assays or co-immunoprecipitation. Additionally, the MPK9 coding sequence and its promoter were cloned into various plant vectors and used to make transgenic plants allowing for in vivo visualization of the subcellular localization of the protein and the expression pattern of the gene. These studies on MPK9 and its partners will work to shed light on its function in the ABA signaling pathway and in the plant as a whole.