Presentation Title

Producing Cytochrome P450 Mutants for Biocatalysis

Faculty Mentor

Andrew Udit

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

HARBESON 15

Session

POSTER 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Abstract:

The purpose of this project is to facilitate a greater understanding of the electrode-bound cytochrome P450 enzyme by producing mutants and using them to understand its behavior at an electrode. The enzyme is involved in the catalysis of numerous bodily reactions including drug responsiveness in humans. However, reproducing this function outside of the body is difficult, given the difficulty of delivering electrons to the enzyme to perform this activity. Though an electrode may help it perform this function, the enzyme does not easily interact with electrodes (Hagen et al., 2013). The project is aimed at producing P450 mutants that are favorable to an electrode, leading to further research on the electrode-bound enzyme. This understanding will be of use to drug research and pharmacokinetics.

Keywords: Cytochrome, Electrode-bound enzyme, Catalysis, Molecular Cloning

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Producing Cytochrome P450 Mutants for Biocatalysis

HARBESON 15

Abstract:

The purpose of this project is to facilitate a greater understanding of the electrode-bound cytochrome P450 enzyme by producing mutants and using them to understand its behavior at an electrode. The enzyme is involved in the catalysis of numerous bodily reactions including drug responsiveness in humans. However, reproducing this function outside of the body is difficult, given the difficulty of delivering electrons to the enzyme to perform this activity. Though an electrode may help it perform this function, the enzyme does not easily interact with electrodes (Hagen et al., 2013). The project is aimed at producing P450 mutants that are favorable to an electrode, leading to further research on the electrode-bound enzyme. This understanding will be of use to drug research and pharmacokinetics.

Keywords: Cytochrome, Electrode-bound enzyme, Catalysis, Molecular Cloning