Presentation Title

Characterization and comparison of human IAPP to IAPPs from Aquatic Mammals

Faculty Mentor

Luiza Nogaj

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

HARBESON 16

Session

POSTER 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Islet Amyloid Polypeptides (IAPPs) are one of the products secreted by pancreatic beta cells. These IAPPs and other amyloid peptides are notorious for aggregating into deposits that destroy beta cells, the primary producers of insulin. IAPP aggregation has been associated with the development of type II diabetes, a disease that affects 30.3 million people of all ages—or 9.4% of the U.S. population, and is an epidemic that has devastated the lives of many. This research explores the relationship between the IAPP sequences, their propensity to aggregate, and their effect on mammalian cell viability. It is unknown whether amyloids are the products of Type 2 diabetes or the cause of it. However, it is known that amyloids exist and that they are detrimental to beta cells. This study focuses on the examination of how IAPP sequence and aggregation propensity in various animals is associated with the prevalence of Type 2 diabetes. Mixed MTT assays were performed to assess cell viability of HeLa cells that were treated with the IAPPs of a Polar Bear, Weddell Seal, and Dolphin. Subsequently, LDH Cytotoxicity assays were conducted to test viability of cellular membrane upon addition of the same IAPPs. The MTT assay results showed that of all species, when animal IAPP was mixed with human IAPP; weddell seal maintained cell viability. Similarly, LDH Cytotoxicity results revealed that when the animal IAPPs were mixed with that of human, the weddell seal samples were not as cytotoxic.

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Characterization and comparison of human IAPP to IAPPs from Aquatic Mammals

HARBESON 16

Islet Amyloid Polypeptides (IAPPs) are one of the products secreted by pancreatic beta cells. These IAPPs and other amyloid peptides are notorious for aggregating into deposits that destroy beta cells, the primary producers of insulin. IAPP aggregation has been associated with the development of type II diabetes, a disease that affects 30.3 million people of all ages—or 9.4% of the U.S. population, and is an epidemic that has devastated the lives of many. This research explores the relationship between the IAPP sequences, their propensity to aggregate, and their effect on mammalian cell viability. It is unknown whether amyloids are the products of Type 2 diabetes or the cause of it. However, it is known that amyloids exist and that they are detrimental to beta cells. This study focuses on the examination of how IAPP sequence and aggregation propensity in various animals is associated with the prevalence of Type 2 diabetes. Mixed MTT assays were performed to assess cell viability of HeLa cells that were treated with the IAPPs of a Polar Bear, Weddell Seal, and Dolphin. Subsequently, LDH Cytotoxicity assays were conducted to test viability of cellular membrane upon addition of the same IAPPs. The MTT assay results showed that of all species, when animal IAPP was mixed with human IAPP; weddell seal maintained cell viability. Similarly, LDH Cytotoxicity results revealed that when the animal IAPPs were mixed with that of human, the weddell seal samples were not as cytotoxic.