Presentation Title

Isolation and Identification of the Molecule Responsible for Resistance to Powdery Mildew Disease

Faculty Mentor

Gregory A. Barding Jr.,

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 11

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Powdery white mildew is a fungal disease that affects a wide variety of plants by leaching away nutrients which effectively weakens the plant, hindering its ability to grow efficiently. The goal for this research project is to isolate and identify a molecule that increases a plant's resistance to powdery white mildew disease. Plant extracts containing the metabolite undergo a series of separation techniques, such as C18 solid phase extraction followed by weak anion exchange solid phase extraction, to isolate the targeted molecule. This molecule is further purified through the use of hydrophilic interaction liquid chromatography in order to separate the polar compounds into fractions. The buffer system used in conjunction with the HPLC utilizes a scale of concentrations to separate the target molecule from the rest of the injected solution. The collected fractions were analyzed with NMR to aid in the identification of the molecule. NMR analysis revealed that the largest bio signaling response was present in the 65% organic buffer eluate. Characterization of this molecule will help provide important information about the fungal disease and could potentially be applied to other agricultural or horticultural projects.

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Nov 17th, 8:30 AM Nov 17th, 10:30 AM

Isolation and Identification of the Molecule Responsible for Resistance to Powdery Mildew Disease

CREVELING 11

Powdery white mildew is a fungal disease that affects a wide variety of plants by leaching away nutrients which effectively weakens the plant, hindering its ability to grow efficiently. The goal for this research project is to isolate and identify a molecule that increases a plant's resistance to powdery white mildew disease. Plant extracts containing the metabolite undergo a series of separation techniques, such as C18 solid phase extraction followed by weak anion exchange solid phase extraction, to isolate the targeted molecule. This molecule is further purified through the use of hydrophilic interaction liquid chromatography in order to separate the polar compounds into fractions. The buffer system used in conjunction with the HPLC utilizes a scale of concentrations to separate the target molecule from the rest of the injected solution. The collected fractions were analyzed with NMR to aid in the identification of the molecule. NMR analysis revealed that the largest bio signaling response was present in the 65% organic buffer eluate. Characterization of this molecule will help provide important information about the fungal disease and could potentially be applied to other agricultural or horticultural projects.