Presentation Title

Effects of Diketopiperazines addition in Marine Bacteria

Faculty Mentor

Jacqueline A. Trischman

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

HARBESON 7

Session

POSTER 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Diketopiperazines (DKPs) are homodetic cyclic dipeptides, including some that are biologically active natural products found in plants, mammals, fungi, and bacteria. DKPs have drawn significant interest due to the diverse range of biological activities in vastly different applications. DKPs and derivatives have been linked to antibacterial activity through the ability to disrupt formation of biofilms through bacterial quorum sensing processes. In this study, 71 marine bacterial strains were selected due to their antibacterial activities in the presence of M. marinum to compare the effects of DKP addition to challenge with the mycobacterium. One DKP compound, cyclo (L-Phe-L-Pro), was synthesized in the laboratory of a collaborator. The marine bacterial strains were placed under different conditions including unchallenged with no treatment (U), or challenged at stationary phase with isopropyl alcohol (IPA) solvent control (S), M. marinum (C), or DKP in IPA solvent (D). A solid-phase extraction (SPE) was performed using three progressively less polar eluting solvents and analyzed by proton NMR. The entire project involves the growth of 284 cultures, with three fractions each (852 in total) needing analysis. Initial results show no significant pattern emerging.

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Effects of Diketopiperazines addition in Marine Bacteria

HARBESON 7

Diketopiperazines (DKPs) are homodetic cyclic dipeptides, including some that are biologically active natural products found in plants, mammals, fungi, and bacteria. DKPs have drawn significant interest due to the diverse range of biological activities in vastly different applications. DKPs and derivatives have been linked to antibacterial activity through the ability to disrupt formation of biofilms through bacterial quorum sensing processes. In this study, 71 marine bacterial strains were selected due to their antibacterial activities in the presence of M. marinum to compare the effects of DKP addition to challenge with the mycobacterium. One DKP compound, cyclo (L-Phe-L-Pro), was synthesized in the laboratory of a collaborator. The marine bacterial strains were placed under different conditions including unchallenged with no treatment (U), or challenged at stationary phase with isopropyl alcohol (IPA) solvent control (S), M. marinum (C), or DKP in IPA solvent (D). A solid-phase extraction (SPE) was performed using three progressively less polar eluting solvents and analyzed by proton NMR. The entire project involves the growth of 284 cultures, with three fractions each (852 in total) needing analysis. Initial results show no significant pattern emerging.