Presentation Title
Detection of Salmonella enterica Contamination in Dog Biscuits Using a DNA-based Biosensor Detection System
Faculty Mentor
Dr. Sylvia A. Vetrone
Start Date
17-11-2018 12:30 PM
End Date
17-11-2018 2:30 PM
Location
HARBESON 8
Session
POSTER 2
Type of Presentation
Poster
Subject Area
biological_agricultural_sciences
Abstract
Our DNA-based biosensor, which utilizes gold nanoparticles (AuNPs) for signal amplification and magnetic nanoparticles (MNPs) for easy and clean separation from samples, has been shown to detect non-PCR amplified genomic DNA targets (DNAt) from bacterial pathogens. While this detection system can provide detection within a five-hour window within liquid food matrices, it needs to be determined how well it will detect pathogenic DNA within solid food matrices. Therefore, in this study genomic DNA was extracted from dog biscuits spiked with Salmonella enterica, E. coli (non-specific negative control), a mixture of Salmonella and E. coli, or just water (negative control) using either Trizol® reagent. Genomic DNA targets (DNAt) from samples at 3ng/mL, 1ng/mL, and 0.125ng/mL were detected using the biosensor: hybridizing the DNAt into a sandwich-like structure consisting of MNPs/DNAt/AuNPs, which were then placed onto screen-printed carbon electrodes to detect the voltammetric peaks of gold using differential pulse voltammetry. Our results indicate that the biosensor is able to detect only the specified pathogenic DNAt, Salmonella enterica, at the higher concentration of 3 ng/mL (p=0.05). These findings suggest that while lower detection limits cannot be achieved within thicker food matrices, this system is still able to detect pathogenic targets within solid food matrices.
Summary of research results to be presented
Our DNA-based biosensor, which utilizes gold nanoparticles (AuNPs) for signal amplification and magnetic nanoparticles (MNPs) for easy and clean separation from samples, has been shown to detect non-PCR amplified genomic DNA targets (DNAt) from bacterial pathogens. While this detection system can provide detection within a five-hour window within liquid food matrices, it needs to be determined how well it will detect pathogenic DNA within solid food matrices. Our Findings suggest that while lower detection limits cannot be achieved within thicker food matrices, this system is still able to detect pathogenic targets within solid food matrices.
Detection of Salmonella enterica Contamination in Dog Biscuits Using a DNA-based Biosensor Detection System
HARBESON 8
Our DNA-based biosensor, which utilizes gold nanoparticles (AuNPs) for signal amplification and magnetic nanoparticles (MNPs) for easy and clean separation from samples, has been shown to detect non-PCR amplified genomic DNA targets (DNAt) from bacterial pathogens. While this detection system can provide detection within a five-hour window within liquid food matrices, it needs to be determined how well it will detect pathogenic DNA within solid food matrices. Therefore, in this study genomic DNA was extracted from dog biscuits spiked with Salmonella enterica, E. coli (non-specific negative control), a mixture of Salmonella and E. coli, or just water (negative control) using either Trizol® reagent. Genomic DNA targets (DNAt) from samples at 3ng/mL, 1ng/mL, and 0.125ng/mL were detected using the biosensor: hybridizing the DNAt into a sandwich-like structure consisting of MNPs/DNAt/AuNPs, which were then placed onto screen-printed carbon electrodes to detect the voltammetric peaks of gold using differential pulse voltammetry. Our results indicate that the biosensor is able to detect only the specified pathogenic DNAt, Salmonella enterica, at the higher concentration of 3 ng/mL (p=0.05). These findings suggest that while lower detection limits cannot be achieved within thicker food matrices, this system is still able to detect pathogenic targets within solid food matrices.