Presentation Title

Investigating Garlic’s time-dependent effects on cytokine secretion from lipopolysaccharide-induced cytokine secretion from macrophage cell lines plated at different cell densities

Faculty Mentor

Nancy E Buckley

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 119

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Garlic (Allium Sativum) has been used as a cure-all in the treatment of ailments for centuries. Of particular interest to our laboratory is the effect that garlic has on cells of the immune system. Macrophages are key immune cells involved in the first line of defense against pathogens, and it is known that garlic can modulate macrophage function. An important immune function of macrophages is the production of cytokines. Macrophages are major producers of the cytokine tumor necrosis factor-alpha (TNF-α). Upon challenging macrophages with lipopolysaccharide (LPS), an outer membrane component of gram-negative bacteria, macrophages are known to secrete copious amounts of TNF-α. In our laboratory, we have found that garlic stimulates LPS-induced TNF-α production from the J774A.1 macrophage cell line, but not from the RAW264.7 macrophage cell line. Thus we are investigating whether this discrepancy is due to cell density and/or time of cell exposure to garlic and/or LPS. To address this, J774A.1 and RAW264.7 are plated at different concentrations (0.625x105-5x105 cells/ml) and treated with garlic (1:500 dilution) and/or LPS (100ng/ml) for different times (6h-24h). After the treatment times, cell supernatants are collected and analyzed for TNF-α using enzyme linked immunosorbent assay. Our preliminary results suggest that garlic’s effects on both macrophage cell lines may be cell density dependent. We are currently confirming these results as well as investigating the time dependent effects. Investigation of the immunomodulatory effects of garlic could lead to potentially undiscovered insight into the mechanisms behind the macrophage response pathway. Further study into foods that can attenuate the immune systems’ response to pathogenic elements can shed some light on the importance of diet.

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Nov 17th, 8:30 AM Nov 17th, 10:30 AM

Investigating Garlic’s time-dependent effects on cytokine secretion from lipopolysaccharide-induced cytokine secretion from macrophage cell lines plated at different cell densities

CREVELING 119

Garlic (Allium Sativum) has been used as a cure-all in the treatment of ailments for centuries. Of particular interest to our laboratory is the effect that garlic has on cells of the immune system. Macrophages are key immune cells involved in the first line of defense against pathogens, and it is known that garlic can modulate macrophage function. An important immune function of macrophages is the production of cytokines. Macrophages are major producers of the cytokine tumor necrosis factor-alpha (TNF-α). Upon challenging macrophages with lipopolysaccharide (LPS), an outer membrane component of gram-negative bacteria, macrophages are known to secrete copious amounts of TNF-α. In our laboratory, we have found that garlic stimulates LPS-induced TNF-α production from the J774A.1 macrophage cell line, but not from the RAW264.7 macrophage cell line. Thus we are investigating whether this discrepancy is due to cell density and/or time of cell exposure to garlic and/or LPS. To address this, J774A.1 and RAW264.7 are plated at different concentrations (0.625x105-5x105 cells/ml) and treated with garlic (1:500 dilution) and/or LPS (100ng/ml) for different times (6h-24h). After the treatment times, cell supernatants are collected and analyzed for TNF-α using enzyme linked immunosorbent assay. Our preliminary results suggest that garlic’s effects on both macrophage cell lines may be cell density dependent. We are currently confirming these results as well as investigating the time dependent effects. Investigation of the immunomodulatory effects of garlic could lead to potentially undiscovered insight into the mechanisms behind the macrophage response pathway. Further study into foods that can attenuate the immune systems’ response to pathogenic elements can shed some light on the importance of diet.