Presentation Title

The IAPP Toxicity of Rat, Raccoon, and Degu on HeLa Cells

Faculty Mentor

Dr. Luiza Nogaj

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 20

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Type 2 diabetes is the most common form of diabetes, where the body suffers from insulin resistance. The islet amyloid polypeptide (IAPP) is a secretory product of beta cells in pancreatic islets of Langerhans that also inhibits glucagon and insulin secretion. The aggregation of IAPP is present in pancreatic islet amyloid deposits seen especially in Type 2 diabetes, in humans and other mammalian species. The objective of this study was to determine the effects of the rodent IAPP toxicity on mammalian cells. In this work, the human IAPP sequence and its molecular effect on mammalian cells was compared to the rodent IAPP sequences and their effect on the cells. Molecular techniques such as MTT and LDH Cytotoxicity were used to test the effect of human and animal IAPP on HeLa cells. The MTT results show a consistent drop in cell viability as the concentration of IAPP from a human, degu, rat and raccoon increases. LDH cytotoxicity shows the highest toxicity in human IAPP compared to the rest of the studied animal IAPPs. Further molecular analysis is necessary to determine the correlation between animal IAPP toxicity and the incidence of Type 2 diabetes.

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Nov 17th, 8:30 AM Nov 17th, 10:30 AM

The IAPP Toxicity of Rat, Raccoon, and Degu on HeLa Cells

CREVELING 20

Type 2 diabetes is the most common form of diabetes, where the body suffers from insulin resistance. The islet amyloid polypeptide (IAPP) is a secretory product of beta cells in pancreatic islets of Langerhans that also inhibits glucagon and insulin secretion. The aggregation of IAPP is present in pancreatic islet amyloid deposits seen especially in Type 2 diabetes, in humans and other mammalian species. The objective of this study was to determine the effects of the rodent IAPP toxicity on mammalian cells. In this work, the human IAPP sequence and its molecular effect on mammalian cells was compared to the rodent IAPP sequences and their effect on the cells. Molecular techniques such as MTT and LDH Cytotoxicity were used to test the effect of human and animal IAPP on HeLa cells. The MTT results show a consistent drop in cell viability as the concentration of IAPP from a human, degu, rat and raccoon increases. LDH cytotoxicity shows the highest toxicity in human IAPP compared to the rest of the studied animal IAPPs. Further molecular analysis is necessary to determine the correlation between animal IAPP toxicity and the incidence of Type 2 diabetes.