Presentation Title

Spatial Differences in the Bacterial Microbiome Associated with Macrocystis Pyrifera across Blade Size and Age

Presenter Information

Emelly OrtizVillaFollow

Faculty Mentor

Diane Kim, Adriane Jones

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 10:30 AM

Location

CREVELING 106

Session

POSTER 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Macrocystis pyrifera is a fast-growing, common macroalgae along the coasts of California. Similarly to how humans depend on a gut microbiome through all developmental stages, it is hypothesized that kelp also rely on their microbiome which can dictate the success of the host. The surface of the kelp supports a diverse microbial community that can change in response to the environment and can aid in nutrient acquisition and carbon exchange (Hans-curt et al., 2016). This project will focus on characterizing differences in the microbiome of blades of varied sizes and developmental stages of growth in addition to successional changes as the kelp develops from spores or vegetative gametophytes. We used a combination of high-throughput 16S rDNA tagged sequencing and cultured isolates to characterize the microbiome of the kelp. Extracting bacterial DNA from macroalgae is difficult since it contains large amounts of alganate that can inhibit extraction and it contains mitochondria and chloroplasts that dominate 16S rDNA libraries. We evaluated three published kelp biofilm DNA extraction methods 1) Shaking whole blades, 2) Scraping blades with a razor blade and 3) Swabbing the blades (Bengtsson et al., 2010;Michelou et al., 2013; Marzinelli et al., 2018). We found that swabbing the blades gave a consistent quantity of DNA and also gave positive results in a 16S rDNA PCR assay. We present a comparison of the bacterial community to analyze the microbial biofilm patterns associated with kelp to show that the microbiome associations are crucial for successful development, and growth.

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Nov 17th, 8:30 AM Nov 17th, 10:30 AM

Spatial Differences in the Bacterial Microbiome Associated with Macrocystis Pyrifera across Blade Size and Age

CREVELING 106

Macrocystis pyrifera is a fast-growing, common macroalgae along the coasts of California. Similarly to how humans depend on a gut microbiome through all developmental stages, it is hypothesized that kelp also rely on their microbiome which can dictate the success of the host. The surface of the kelp supports a diverse microbial community that can change in response to the environment and can aid in nutrient acquisition and carbon exchange (Hans-curt et al., 2016). This project will focus on characterizing differences in the microbiome of blades of varied sizes and developmental stages of growth in addition to successional changes as the kelp develops from spores or vegetative gametophytes. We used a combination of high-throughput 16S rDNA tagged sequencing and cultured isolates to characterize the microbiome of the kelp. Extracting bacterial DNA from macroalgae is difficult since it contains large amounts of alganate that can inhibit extraction and it contains mitochondria and chloroplasts that dominate 16S rDNA libraries. We evaluated three published kelp biofilm DNA extraction methods 1) Shaking whole blades, 2) Scraping blades with a razor blade and 3) Swabbing the blades (Bengtsson et al., 2010;Michelou et al., 2013; Marzinelli et al., 2018). We found that swabbing the blades gave a consistent quantity of DNA and also gave positive results in a 16S rDNA PCR assay. We present a comparison of the bacterial community to analyze the microbial biofilm patterns associated with kelp to show that the microbiome associations are crucial for successful development, and growth.