Presentation Title

In-vivo and In-vitro Evaluation of Enzymes of Human Vaginal Matrices

Faculty Mentor

Manjula Gunawardana M.P.H.

Start Date

17-11-2018 9:45 AM

End Date

17-11-2018 10:00 AM

Location

C161

Session

Oral 2

Type of Presentation

Oral Talk

Subject Area

biological_agricultural_sciences

Abstract

In-vivo and In-vitro Evaluation of Enzymes of Human Vaginal Matrices.

Author: Ivan Villanueva, University of California, Irvine.

Sylvia Pere, Citrus College.

Mentor: Manjula Gunawardana, Oak Crest Institute of Science.

Hydrolases are biochemical catalysts that use water to cleave chemical bonds, usually process a large molecule into two smaller molecules. We analyzed and quantified the presence of hydrolytic enzymes (esterase, protease, neuraminidase and amylase) in the vaginal epithelium using tissue culture and clinical samples. Understanding enzymatic activity in the female reproductive system helps improve delivery of therapeutics through intravaginal rings (IVR)- a female controlled strategy that can deliver drugs intra-vaginally to protect women from HIV, other infections, as well as unintended pregnancy. We compared enzyme specific activity between female volunteers (IRB OCIS-002) and tissue culture (in-situ) samples of the Vaginal Epithelial Cells (VEC). Comparison of matrices indicated significant esterase activity in in-situ samples; and higher protease, neuraminidase and amylase activity in human samples. Our findings will contribute to research in dosage determination of topical therapeutics enabling higher efficacy.

Keywords: Protease, Neuraminidase, Amylase, Esterase, HIV prevention, Intravaginal ring, Enzymes.

Summary of research results to be presented

Results

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Nov 17th, 9:45 AM Nov 17th, 10:00 AM

In-vivo and In-vitro Evaluation of Enzymes of Human Vaginal Matrices

C161

In-vivo and In-vitro Evaluation of Enzymes of Human Vaginal Matrices.

Author: Ivan Villanueva, University of California, Irvine.

Sylvia Pere, Citrus College.

Mentor: Manjula Gunawardana, Oak Crest Institute of Science.

Hydrolases are biochemical catalysts that use water to cleave chemical bonds, usually process a large molecule into two smaller molecules. We analyzed and quantified the presence of hydrolytic enzymes (esterase, protease, neuraminidase and amylase) in the vaginal epithelium using tissue culture and clinical samples. Understanding enzymatic activity in the female reproductive system helps improve delivery of therapeutics through intravaginal rings (IVR)- a female controlled strategy that can deliver drugs intra-vaginally to protect women from HIV, other infections, as well as unintended pregnancy. We compared enzyme specific activity between female volunteers (IRB OCIS-002) and tissue culture (in-situ) samples of the Vaginal Epithelial Cells (VEC). Comparison of matrices indicated significant esterase activity in in-situ samples; and higher protease, neuraminidase and amylase activity in human samples. Our findings will contribute to research in dosage determination of topical therapeutics enabling higher efficacy.

Keywords: Protease, Neuraminidase, Amylase, Esterase, HIV prevention, Intravaginal ring, Enzymes.