Presentation Title

The Effects of Alpha-Lipoic Acid on Lactate Dehydrogenase Enzyme Kinetics

Faculty Mentor

Jerome Garcia

Start Date

17-11-2018 10:15 AM

End Date

17-11-2018 10:30 AM

Location

C161

Session

Oral 2

Type of Presentation

Oral Talk

Subject Area

biological_agricultural_sciences

Abstract

Alpha-lipoic acid (ALA) is a coenzyme naturally produced in the human body that permits various metabolic functions. ALA is sold commercially as a result of past studies on how this supplement induces weight loss. In our laboratory, previous studies have demonstrated that ALA inhibits lactate dehydrogenase (LDH), an oxidoreductase catalytic enzyme, from converting pyruvate into lactic acid (unpublished data). The inhibition effect that ALA has on LDH is a possible mechanism to which weight loss occurs. The present study further investigates how ALA effects LDH enzyme kinetics in transforming lactic acid back into pyruvate. Crude liver homogenates were generated from livers obtained from C57BL/6J mice. LDH enzymatic activity was measured by analyzing NADH formation using a spectrophotometer at 340 nm. Collected data indicated that NADH formation plateaus at 5 mM untreated lactate indicating saturation of LDH. A series of ALA treatments, varying in concentration, were then performed on 5 mM lactate and NADH formation was observed using a spectrophotometer at 340 nm. The results were consistent for all three livers, and concluded that ALA treatment (of all concentrations) decreased the production of NADH concentrations (uM/sL) in 5 mM lactate samples.

Summary of research results to be presented

Lactate dehydrogenase (LDH) enzymatic activity was observed by measuring the NADH production with a spectrophotometer. 5 mM lactate was used based on the enzyme kinetic curve. Alpha-lipoic acid (ALA) was then administered, at various concentrations, to observe the inhibitory effects. Our results concluded that all concentrations of ALA treatment have decreased NADH production and reduced LDH enzymatic activity.

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Nov 17th, 10:15 AM Nov 17th, 10:30 AM

The Effects of Alpha-Lipoic Acid on Lactate Dehydrogenase Enzyme Kinetics

C161

Alpha-lipoic acid (ALA) is a coenzyme naturally produced in the human body that permits various metabolic functions. ALA is sold commercially as a result of past studies on how this supplement induces weight loss. In our laboratory, previous studies have demonstrated that ALA inhibits lactate dehydrogenase (LDH), an oxidoreductase catalytic enzyme, from converting pyruvate into lactic acid (unpublished data). The inhibition effect that ALA has on LDH is a possible mechanism to which weight loss occurs. The present study further investigates how ALA effects LDH enzyme kinetics in transforming lactic acid back into pyruvate. Crude liver homogenates were generated from livers obtained from C57BL/6J mice. LDH enzymatic activity was measured by analyzing NADH formation using a spectrophotometer at 340 nm. Collected data indicated that NADH formation plateaus at 5 mM untreated lactate indicating saturation of LDH. A series of ALA treatments, varying in concentration, were then performed on 5 mM lactate and NADH formation was observed using a spectrophotometer at 340 nm. The results were consistent for all three livers, and concluded that ALA treatment (of all concentrations) decreased the production of NADH concentrations (uM/sL) in 5 mM lactate samples.