Presentation Title

The Effect of Essiac Tea's Antioxidant Properties on in vitro and in vivo Models

Faculty Mentor

Sylvia Vetrone

Start Date

17-11-2018 8:15 AM

End Date

17-11-2018 8:30 AM

Location

C302

Session

Oral 1

Type of Presentation

Oral Talk

Subject Area

biological_agricultural_sciences

Abstract

Essiac tea is an antioxidant that is touted as a homeopathic cancer treatment, which contains four herbs: Arctium lappa, Rumex acetosella, Ulmus rubra and Rheum officinale. As previous studies have demonstrated Essiac’s antioxidant properties to combat DNA damage by reactive oxygen species (ROS), we hypothesized that Essiac tea would also have the capacity to boost an organism’s immune system, by reducing ROS and therefore positively affect life span. Accordingly, our study investigated Essiac’s antioxidant properties on lifespan and oxidative stress resistance in the nematode model, Caenorabditis elegans (C. elegans). Similar to previous findings, we found that C. elegans treated with a 100% of the recommended dosage of Essiac tea had a statistical increase in their overall lifespan, and ability to withstand acute oxidative stressed when compared to their control treated group. Additionally, we hypothesized that Essiac’s antioxidant properties would also be able to inhibit cell proliferation in both suspension and tumor forming cell lines. Using three viability and cytotoxicity assays, our work demonstrated that a 12% exposure of Essiac tea induced a significant increase in cell death and reduced viability within five cancer cell lines (RPMI 8226, Jurkat, CML, LNCAP, and MCF7). As our in vitro and in vivo findings are very promising, current studies are exploring the mechanism through which Essiac tea is exerting its effects on both C. elegans and cancer cell lines.

Summary of research results to be presented

We hypothesized that Essiac tea’s antioxidant properties could help boost the immune response and life span of the nematode animal model, Caenorhabditis elegans (C. elegans). The results showed that exposure of 100% dosage of Essiac correlated with improvements to C. elegans. C. elegans exposed to a 100% dosage of Essiac not only demonstrated a statistically longer lifespan, but also a higher viability rate when exposed to oxidative stress

Additionally, we also hypothesized that cancer cells (both suspended and tumor forming) exposed to Essiac would exhibit reduced cell proliferation due to its antioxidant properties. Trypan blue viability assay demonstrated RMPI 8226 cells (B cell myeloma) exposed to concentrations ranging from 6.25% 50% had statistically reduction in viability compared to their control group. These results were also confirmed through a second viability assay, Cell Titer96 Assay using four additional cell lines, Jurkats and CMLs, along with tumor forming LNCAPs and MCF7s. Exposure to Essiac for both 48 and 72 hours resulted in reduced viability for all cells treated with at minimum 12% Essiac at 48 hours, and 6% at 72 hours.

Taken together, our findings are very promising in demonstrating the health and anti-cancer properties of Essiac. Current studies are on-going to determine the mechanisms in which Essiac tea is exerting its antioxidant and anti-proliferative effects on both C. elegans and cancer cell lines.

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Nov 17th, 8:15 AM Nov 17th, 8:30 AM

The Effect of Essiac Tea's Antioxidant Properties on in vitro and in vivo Models

C302

Essiac tea is an antioxidant that is touted as a homeopathic cancer treatment, which contains four herbs: Arctium lappa, Rumex acetosella, Ulmus rubra and Rheum officinale. As previous studies have demonstrated Essiac’s antioxidant properties to combat DNA damage by reactive oxygen species (ROS), we hypothesized that Essiac tea would also have the capacity to boost an organism’s immune system, by reducing ROS and therefore positively affect life span. Accordingly, our study investigated Essiac’s antioxidant properties on lifespan and oxidative stress resistance in the nematode model, Caenorabditis elegans (C. elegans). Similar to previous findings, we found that C. elegans treated with a 100% of the recommended dosage of Essiac tea had a statistical increase in their overall lifespan, and ability to withstand acute oxidative stressed when compared to their control treated group. Additionally, we hypothesized that Essiac’s antioxidant properties would also be able to inhibit cell proliferation in both suspension and tumor forming cell lines. Using three viability and cytotoxicity assays, our work demonstrated that a 12% exposure of Essiac tea induced a significant increase in cell death and reduced viability within five cancer cell lines (RPMI 8226, Jurkat, CML, LNCAP, and MCF7). As our in vitro and in vivo findings are very promising, current studies are exploring the mechanism through which Essiac tea is exerting its effects on both C. elegans and cancer cell lines.