Presentation Title

Synthesis of polarization agents for NMR spectroscopy

Faculty Mentor

Galia Debelouchina

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

CREVELING 21

Session

POSTER 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

In this research, organic chemistry methods are used to synthesize the polarizing reagent 1-(TEMPO-4-oxy)-3-(TEMPO-4-amino)propan-2-ol (TOTAPOL), and the reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid. These reagents will be used congruently with Dynamic Nuclear Polarization NMR spectroscopy to intensify NMR signals of biological proteins. DNP-NMR requires the use of a polarizing reagent to increase proton signals, to satisfy this condition the biradical TOTAPOL is used due it its efficiency of transferring electron polarization to surrounding protons. The reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid is utilized as a linker to form a bond between the protein and the polarizing reagent. Collectively, this research aims to incorporate synthetic chemistry and chemical biology methods to increase the intensity of NMR signals of biological proteins in order to characterize their structure.

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Synthesis of polarization agents for NMR spectroscopy

CREVELING 21

In this research, organic chemistry methods are used to synthesize the polarizing reagent 1-(TEMPO-4-oxy)-3-(TEMPO-4-amino)propan-2-ol (TOTAPOL), and the reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid. These reagents will be used congruently with Dynamic Nuclear Polarization NMR spectroscopy to intensify NMR signals of biological proteins. DNP-NMR requires the use of a polarizing reagent to increase proton signals, to satisfy this condition the biradical TOTAPOL is used due it its efficiency of transferring electron polarization to surrounding protons. The reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid is utilized as a linker to form a bond between the protein and the polarizing reagent. Collectively, this research aims to incorporate synthetic chemistry and chemical biology methods to increase the intensity of NMR signals of biological proteins in order to characterize their structure.