Presentation Title

Synthesis of polarization agents for NMR spectroscopy

Faculty Mentor

Galia Debelouchina

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

CREVELING 21

Session

POSTER 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

In this research, organic chemistry methods are used to synthesize the polarizing reagent 1-(TEMPO-4-oxy)-3-(TEMPO-4-amino)propan-2-ol (TOTAPOL), and the reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid. These reagents will be used congruently with Dynamic Nuclear Polarization NMR spectroscopy to intensify NMR signals of biological proteins. DNP-NMR requires the use of a polarizing reagent to increase proton signals, to satisfy this condition the biradical TOTAPOL is used due it its efficiency of transferring electron polarization to surrounding protons. The reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid is utilized as a linker to form a bond between the protein and the polarizing reagent. Collectively, this research aims to incorporate synthetic chemistry and chemical biology methods to increase the intensity of NMR signals of biological proteins in order to characterize their structure.

Summary of research results to be presented

It has been shown that when the polarization agent is dissolved in solution with biological proteins, NMR signals have increased intensity.The polarization agent was synthesized with a 74% yield. Along with the polarization agent, different linking molecules were synthesized at 28.3 % and at 20 % yield. Portions of the polarization agent and the linking molecule were then combined into one molecule at 28 % and 7.34 %. Further work is to come, the goal of the lab is to attach the combined molecule onto a biological protein and compare the intensity of NMR signals to the NMR signals received when the polarization agent was in solution.

This document is currently not available here.

Share

COinS
 
Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Synthesis of polarization agents for NMR spectroscopy

CREVELING 21

In this research, organic chemistry methods are used to synthesize the polarizing reagent 1-(TEMPO-4-oxy)-3-(TEMPO-4-amino)propan-2-ol (TOTAPOL), and the reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid. These reagents will be used congruently with Dynamic Nuclear Polarization NMR spectroscopy to intensify NMR signals of biological proteins. DNP-NMR requires the use of a polarizing reagent to increase proton signals, to satisfy this condition the biradical TOTAPOL is used due it its efficiency of transferring electron polarization to surrounding protons. The reagent 3,3’-(1,2,4,5-tetrazine)dibenzoic acid is utilized as a linker to form a bond between the protein and the polarizing reagent. Collectively, this research aims to incorporate synthetic chemistry and chemical biology methods to increase the intensity of NMR signals of biological proteins in order to characterize their structure.