Presentation Title

Synthesis of Ethynylcobaltocinium for Biocatalysis with P450 Enzymes

Faculty Mentor

Andrew Udit

Start Date

17-11-2018 12:30 PM

End Date

17-11-2018 2:30 PM

Location

CREVELING 26

Session

POSTER 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Cytochrome P450 enzymes (P450) have the potential to be useful in many industries, including applications in pharmaceuticals, pesticides, fragrances and more. P450’s catalytic cycle is reliant on NAD(P)H to function which is expensive and degrades during long term storage. Ultimately, we seek to replace NAD(P)H with a comparable, cheaper and more effective system using cobaltocenium derivatives as substitutes. Our cobaltocenium product functions as a mediator is to transfer electrons from an electrode to the protein in order to create a new and complete electrochemical system. This project is made up of four parts. First, we synthesize our cobaltocenium derivatives. Then we alter our protein and quantify the synthesized protein. The protein development consists of replacing the naturally occuring thiol group with an azide group. Then the cobaltocenium derivative is attached via a “click” reaction. Quantification is done using ICP, UV-Vis, and fluorometric analysis. Finally electrochemical testing and catalytic testing test the effectiveness of the P450-cobaltocenium as an electron mediator.

Summary of research results to be presented

Our research has found strong results indicating the potential of P450-Co as an enzyme to catalyze the same reactions as P450 in the body. The quantification has shown that P450 can be easily and efficiently attached to the cobaltocenium derivative via a click reaction. We have also performed electrochemical testing which demonstrates that the cobalticenium is an effective electron mediator. This indicates that the P450-Co can be used as a successful catalyst.

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Nov 17th, 12:30 PM Nov 17th, 2:30 PM

Synthesis of Ethynylcobaltocinium for Biocatalysis with P450 Enzymes

CREVELING 26

Cytochrome P450 enzymes (P450) have the potential to be useful in many industries, including applications in pharmaceuticals, pesticides, fragrances and more. P450’s catalytic cycle is reliant on NAD(P)H to function which is expensive and degrades during long term storage. Ultimately, we seek to replace NAD(P)H with a comparable, cheaper and more effective system using cobaltocenium derivatives as substitutes. Our cobaltocenium product functions as a mediator is to transfer electrons from an electrode to the protein in order to create a new and complete electrochemical system. This project is made up of four parts. First, we synthesize our cobaltocenium derivatives. Then we alter our protein and quantify the synthesized protein. The protein development consists of replacing the naturally occuring thiol group with an azide group. Then the cobaltocenium derivative is attached via a “click” reaction. Quantification is done using ICP, UV-Vis, and fluorometric analysis. Finally electrochemical testing and catalytic testing test the effectiveness of the P450-cobaltocenium as an electron mediator.