Presentation Title

Paper-Based Microfluidic Devices for Glucose Assays Employing a Metal-Organic Framework (MOF)

Faculty Mentor

Yangyang Liu, Frank A. Gomez

Start Date

17-11-2018 8:30 AM

End Date

17-11-2018 8:45 AM

Location

C323

Session

Oral 1

Type of Presentation

Oral Talk

Subject Area

physical_mathematical_sciences

Abstract

This project describes the development of two microfluidic paper-based analytical devices (μPADs), one well-based and the other based on a lateral flow assay (LFA) configuration, to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Fe-PCN-222, to encapsulate glucose oxidase (GOx). The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx-MOF species sandwiched between the paper layers realizing a yellow-brown color. The LFA platform consisted of chromatography paper between parafilm and polyvinyl acetate (PVA) layers. MOFs spotted on the paper subjected to solutions of KI and glucose yielded a brown color. The devices were then dried, scanned, and analyzed yielding a correlation between average inverse yellow intensity and glucose concentrations. The development of these devices employing MOFs as biomimetic catalysts should further expand the applications of microfluidic technologies as sensors for a variety of analytes.

Summary of research results to be presented

We have described well-based and LFA-based μPAD platforms that utilize the porphyrinic MOF, Zr-PCN-222 (Fe), with encapsulation of GOx for viable performance of a glucose assay via colorimetric detection. For both LFA-based and well-based platforms, glucose detection obeys the Michaelis-Menten enzyme kinetics, however the limits of detection vary between the platforms. Future work is focused on the ability to quantify the rate at which fluids diffuse into Fe-PCN-222 for a better understanding of the color formation taking a minimum of twice as long as previously reported enzyme assays. Future studies are also focused on optimizing the platforms to increase turnover efficiency (Kcat).

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Nov 17th, 8:30 AM Nov 17th, 8:45 AM

Paper-Based Microfluidic Devices for Glucose Assays Employing a Metal-Organic Framework (MOF)

C323

This project describes the development of two microfluidic paper-based analytical devices (μPADs), one well-based and the other based on a lateral flow assay (LFA) configuration, to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Fe-PCN-222, to encapsulate glucose oxidase (GOx). The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx-MOF species sandwiched between the paper layers realizing a yellow-brown color. The LFA platform consisted of chromatography paper between parafilm and polyvinyl acetate (PVA) layers. MOFs spotted on the paper subjected to solutions of KI and glucose yielded a brown color. The devices were then dried, scanned, and analyzed yielding a correlation between average inverse yellow intensity and glucose concentrations. The development of these devices employing MOFs as biomimetic catalysts should further expand the applications of microfluidic technologies as sensors for a variety of analytes.