Presentation Title

DNA Origami-Origami: A Pathway to Multi Drirectional DNA Tessellations

Presenter Information

John GarciaFollow

Faculty Mentor

Dr. Jillian Blatti

Start Date

17-11-2018 3:00 PM

End Date

17-11-2018 5:00 PM

Location

CREVELING 68

Session

POSTER 3

Type of Presentation

Poster

Subject Area

behavioral_social_sciences

Abstract

Although DNA nanotechnology has made many significant advances since it was first introduced by Paul Rothemund in 2006, there is still room to expand the capabilities of DNA Origami. Inspired by the art of origami, we set out to develop a method by which one can achieve actual origami with DNA nanostructures. The term DNA origami is slightly misleading, as it does not follow the same rules as the Japanese artform. The term is used to describe the general ideal that DNA is folded into nanostructures, but the process bears little resemblance to the actual art of folding paper. The DNA nanostructures are designed using a program called caDNAno, which allows one to design complimentary oligomers of DNA that will fold the DNA plasmid template into a shape. Thus, one of the limiting factors is the size of the plasmid template. Reasoning that a modular design could be rendered into any shape with no limitations, initially, the plan was to design a tessellated DNA origami sheet that could be controlled by an electric field. Encountering numerous challenges with this original plan, it was decided to use a combination of 3D and 2D origami nanostructures to create large tessellated structures of DNA whose orientation can be dictated through design of the joints that connect the smaller DNA structures.

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Nov 17th, 3:00 PM Nov 17th, 5:00 PM

DNA Origami-Origami: A Pathway to Multi Drirectional DNA Tessellations

CREVELING 68

Although DNA nanotechnology has made many significant advances since it was first introduced by Paul Rothemund in 2006, there is still room to expand the capabilities of DNA Origami. Inspired by the art of origami, we set out to develop a method by which one can achieve actual origami with DNA nanostructures. The term DNA origami is slightly misleading, as it does not follow the same rules as the Japanese artform. The term is used to describe the general ideal that DNA is folded into nanostructures, but the process bears little resemblance to the actual art of folding paper. The DNA nanostructures are designed using a program called caDNAno, which allows one to design complimentary oligomers of DNA that will fold the DNA plasmid template into a shape. Thus, one of the limiting factors is the size of the plasmid template. Reasoning that a modular design could be rendered into any shape with no limitations, initially, the plan was to design a tessellated DNA origami sheet that could be controlled by an electric field. Encountering numerous challenges with this original plan, it was decided to use a combination of 3D and 2D origami nanostructures to create large tessellated structures of DNA whose orientation can be dictated through design of the joints that connect the smaller DNA structures.