Presentation Title

The Influence of Prebiotic & Probiotic Supplementation on the Oral Microbiome

Faculty Mentor

Marcella Olave

Start Date

18-11-2017 9:45 AM

End Date

18-11-2017 10:00 AM

Location

9-255

Session

Bio Sciences 1

Type of Presentation

Oral Talk

Subject Area

health_nutrition_clinical_science

Abstract

Previous studies have demonstrated that prebiotics and probiotics can have an influence on the health of the human gut microbiome. This has led some to speculate that using prebiotics and probiotics might also have a positive influence on the health of the oral microbiome. The purpose of this study was to determine whether the uses of prebiotics and probiotics, in the case of this study Microbiome Plus+ Gastrointestinal Prebiotic/Probiotic Fiber, have an influence on the reduction of the harmful bacteria—specifically Actinobacillus actinomycetemcomitans—in the oral cavity. Twenty-one individuals participated in this study. The participants were divided into three groups of seven: one received prebiotics, another probiotics, and the third group received no supplement, so that it could serve as the control group for the study. The overall oral health of each individual was observed over a three-week period. After the three weeks, the result of each participant after taking the treatment was compared to the result of the same participant before taking the treatment. This comparison served to determine how the ratio of the colonies of disease-associated organisms (Actinobacillus actinomycetemcomitans) changed before and after putting the participants on the intake. The result indicates that the intake of probiotic supplements has a significant effect in the improvement of oral health by reduction of the growth of Actinobacillus actinomycetemcomitans over a period of three weeks. However, there are several factors to take in consideration for further studies, such as longer duration and diet.

Summary of research results to be presented

This study intended to examine how the use of a prebiotic or a probiotic could influence the oral microbiome. Seven participants were assigned to take prebiotic while seven other participants were assigned to take probiotic. Within the duration of three weeks, pre-sampling and post-sampling tests were performed from several areas of the oral cavity, and plated on a selective medium to observe and analyze the growth of Actinobacillus actinomycetemcomitan. The colonies of Actinobacillus actinomycetemcomitans, from each of the 21 participants, were counted before and after the treatment (Microbiome Plus+ Gastrointestinal Prebiotic/Probiotic Fiber). The prebiotic and probiotic groups had a majority of participants with a decrease in colonies, which may indicate that the treatments may have had a beneficial effect on the health of the oral cavity. In particular, five participants in the prebiotic group and six participants in the probiotic group experienced a decrease in the number of colonies of Actinobacillus actinomycetemcomitans. The percentage of change for each participant ranged from -5.34% to -85.56%. Performing a paired t-test for before and after given data of each group gave the p-value of 0.057 for the prebiotic group (0.057 > 0.05), which indicates that the prebiotic group did not show significant results in this research. The p-value of probiotic was 0.025 (0.025< 0.05) indicating that the probiotic group showed significant beneficial results. Further, the control group had a p-value of 0.207, which means that there was not a significant difference for the control group.

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Nov 18th, 9:45 AM Nov 18th, 10:00 AM

The Influence of Prebiotic & Probiotic Supplementation on the Oral Microbiome

9-255

Previous studies have demonstrated that prebiotics and probiotics can have an influence on the health of the human gut microbiome. This has led some to speculate that using prebiotics and probiotics might also have a positive influence on the health of the oral microbiome. The purpose of this study was to determine whether the uses of prebiotics and probiotics, in the case of this study Microbiome Plus+ Gastrointestinal Prebiotic/Probiotic Fiber, have an influence on the reduction of the harmful bacteria—specifically Actinobacillus actinomycetemcomitans—in the oral cavity. Twenty-one individuals participated in this study. The participants were divided into three groups of seven: one received prebiotics, another probiotics, and the third group received no supplement, so that it could serve as the control group for the study. The overall oral health of each individual was observed over a three-week period. After the three weeks, the result of each participant after taking the treatment was compared to the result of the same participant before taking the treatment. This comparison served to determine how the ratio of the colonies of disease-associated organisms (Actinobacillus actinomycetemcomitans) changed before and after putting the participants on the intake. The result indicates that the intake of probiotic supplements has a significant effect in the improvement of oral health by reduction of the growth of Actinobacillus actinomycetemcomitans over a period of three weeks. However, there are several factors to take in consideration for further studies, such as longer duration and diet.