Presentation Title

Bromelia pinguin Aerial Root Extract Induces HeLa Cell Apoptosis: MTT and Cytotoxicity Assay-Supported

Faculty Mentor

Sylvine Deprèle, PhD, Luiza Nogaj, PhD

Start Date

18-11-2017 9:30 AM

End Date

18-11-2017 9:45 AM

Location

9-279

Session

Physical Sciences 1

Type of Presentation

Oral Talk

Subject Area

physical_mathematical_sciences

Abstract

Native to Sinaloa, Mexico and found in Latin America, Bromelia pingiun L. is a plant that grows a fruit known as aguama, piñuela, or timbiriche. Recent studies have found that B. pinguin’s fruit carries antibacterial activity, antifungal activity, and has a potential as a functional food – providing health benefits. Recent studies have suggested that the fruit has anticancer properties; however, little research has been conducted on B. pinguin’s roots, let alone its effects on mammalian cells.

The objective of this research focuses on B. pinguin’s aerial roots’ anticancer potential by testing three concentrations (0.5%, 1%, and 2%) of its extract on HeLa cells. The extract was obtained after a 4-hour continuous soxhlet extraction using 11 grams of the plant’s aerial root and 150 mL of ethanol as the solvent. The MTT and cytotoxicity assays were conducted to test cytotoxicity and cell viability in exposure to the B. pinguin’s aerial root extract. MTT assay results verified that the B. pinguin extract dramatically decreased HeLa cell viability to 35% at 0.5% concentration in comparison to the control of 100% cell viability. The cytotoxicity assay also followed an increasing trend in cytotoxicity levels as extract concentrations increased, resulting in 10.33% cytotoxicity at the highest concentration of 2%. In the future, B. pinguin’s aerial roots will be extracted using a different solvent system and the effects of those extracts on mammalian cells will be tested. The MTT assay and cytotoxicity assay will be completed again and the extract composition will be analyzed using the GCMS.

Summary of research results to be presented

MTT assay results verified that the B. pinguin extract dramatically decreased HeLa cell viability to 35% at 0.5% concentration in comparison to the control of 100% cell viability. The cytotoxicity assay also followed an increasing trend in cytotoxicity levels as extract concentrations increased, resulting in 10.33% cytotoxicity at the highest concentration of 2%.

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Nov 18th, 9:30 AM Nov 18th, 9:45 AM

Bromelia pinguin Aerial Root Extract Induces HeLa Cell Apoptosis: MTT and Cytotoxicity Assay-Supported

9-279

Native to Sinaloa, Mexico and found in Latin America, Bromelia pingiun L. is a plant that grows a fruit known as aguama, piñuela, or timbiriche. Recent studies have found that B. pinguin’s fruit carries antibacterial activity, antifungal activity, and has a potential as a functional food – providing health benefits. Recent studies have suggested that the fruit has anticancer properties; however, little research has been conducted on B. pinguin’s roots, let alone its effects on mammalian cells.

The objective of this research focuses on B. pinguin’s aerial roots’ anticancer potential by testing three concentrations (0.5%, 1%, and 2%) of its extract on HeLa cells. The extract was obtained after a 4-hour continuous soxhlet extraction using 11 grams of the plant’s aerial root and 150 mL of ethanol as the solvent. The MTT and cytotoxicity assays were conducted to test cytotoxicity and cell viability in exposure to the B. pinguin’s aerial root extract. MTT assay results verified that the B. pinguin extract dramatically decreased HeLa cell viability to 35% at 0.5% concentration in comparison to the control of 100% cell viability. The cytotoxicity assay also followed an increasing trend in cytotoxicity levels as extract concentrations increased, resulting in 10.33% cytotoxicity at the highest concentration of 2%. In the future, B. pinguin’s aerial roots will be extracted using a different solvent system and the effects of those extracts on mammalian cells will be tested. The MTT assay and cytotoxicity assay will be completed again and the extract composition will be analyzed using the GCMS.