Presentation Title

A Microfluidic Glucose Sensor Incorporating a Novel Thread-Based Electrode System

Faculty Mentor

Dr. Frank Gomez

Start Date

18-11-2017 11:00 AM

End Date

18-11-2017 11:15 AM

Location

9-263

Session

Physical Sciences 3

Type of Presentation

Oral Talk

Subject Area

physical_mathematical_sciences

Abstract

An electrochemical sensor for the detection of glucose using thread-based electrodes and fabric is described. Fabrication of the device consisted of two steps. Three thread-based electrodes (reference, working, and counter) were fabricated by painting pieces of nylon thread with either layered silver ink and carbon ink or silver/silver chloride ink. The threads were then woven into a fabric chip with a beeswax barrier molded around the edges in order to prevent leaks from the solution dropped onto the chip. This solution was composed of potassium ferricyanide ([Fe(CN)6]) (as mediator for the enzyme), glucose oxidase (GOx), and increasing concentrations of glucose in buffer added to the top of the chip. The oxidation of glucose by GOx was measured to determine the concentration of glucose in the solution. Using cyclic voltammetry (CV) a scanning voltage of 0-0.35 V was applied. A graph of voltage applied (V) vs. current output (Amps) yielded oxidation and reduction peaks, and this voltage range was then used to run linear scans. The concentration of glucose in the samples was proportional to the current output and was observed as an increased peak height of the oxidation peak. A concentration of 10 mg/mL of both mediator and enzyme was determined to be the most optimal for glucose concentrations of 0-25mM. The current output from the oxidation peak at 0.3V was recorded for each glucose concentration, and a linear curve of glucose concentration vs. current output (run in triplicate) yielded an R2 value of 0.993. This device is relatively simple to fabricate and can be used for multiple readings after washing with ethanol.

This document is currently not available here.

Share

COinS
 
Nov 18th, 11:00 AM Nov 18th, 11:15 AM

A Microfluidic Glucose Sensor Incorporating a Novel Thread-Based Electrode System

9-263

An electrochemical sensor for the detection of glucose using thread-based electrodes and fabric is described. Fabrication of the device consisted of two steps. Three thread-based electrodes (reference, working, and counter) were fabricated by painting pieces of nylon thread with either layered silver ink and carbon ink or silver/silver chloride ink. The threads were then woven into a fabric chip with a beeswax barrier molded around the edges in order to prevent leaks from the solution dropped onto the chip. This solution was composed of potassium ferricyanide ([Fe(CN)6]) (as mediator for the enzyme), glucose oxidase (GOx), and increasing concentrations of glucose in buffer added to the top of the chip. The oxidation of glucose by GOx was measured to determine the concentration of glucose in the solution. Using cyclic voltammetry (CV) a scanning voltage of 0-0.35 V was applied. A graph of voltage applied (V) vs. current output (Amps) yielded oxidation and reduction peaks, and this voltage range was then used to run linear scans. The concentration of glucose in the samples was proportional to the current output and was observed as an increased peak height of the oxidation peak. A concentration of 10 mg/mL of both mediator and enzyme was determined to be the most optimal for glucose concentrations of 0-25mM. The current output from the oxidation peak at 0.3V was recorded for each glucose concentration, and a linear curve of glucose concentration vs. current output (run in triplicate) yielded an R2 value of 0.993. This device is relatively simple to fabricate and can be used for multiple readings after washing with ethanol.