Presentation Title

Quantification of Mitragynine from Kratom Leaves (an Alternative Recreational Drug) with Efficient Extraction Method

Presenter Information

Taehoon HaFollow
Karno Ng, CSUSMFollow

Faculty Mentor

Karno Ng

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 122

Session

Poster 1

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Mitragyna speciosa, also known as Kratom, is an evergreen tree native to Southeast Asia that has been used to relieve pain. The leaves can be chewed, processed into capsular tablet, made into tea, and occasionally smoked. The primary active alkaloid, mitragynine, found in Kratom affects the same receptor as opioids such as heroine. The use of Kratom as an alternative recreational drug has been increasing. Depending on the dosage, consumption of Kratom can lead to psychotic symptoms and various effects. Consequently, the potential effects of Kratom on humans draw international concerns. The purpose of this study is to develop an efficient extraction method for mitragynine from dried Kratom. Grounded Kratom leaves were soaked in methanol and sonicated with a mixture of methanol and chloroform to yield Kratom extract that was rich in alkaloid. The pH of the Kratom extract was adjusted to 9.5 by sodium carbonate solution. The alkaloids were subsequently extracted into chloroform from the basified solution and the resulting dried residue was re-dissolved in 4.0 mL of methanol and then filtered through a 0.45 µm syringe filter prior to injection to reversed-phase HPLC with a C-18 column. The sample was eluted isocratically with a mobile phase of 0.05% formic acid and acetonitrile at pH 5.0 (50:50, v/v), flow rate = 1.0 mL/min. Mitragynine was detected at 223 nm with a retention time of 2.0 min. A calibration curve was prepared with mitragynine standards. Linear correlation was excellent (R2 ­=0.9987). The amount of mitragynine in Kratom was determined (mg/g).

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

Quantification of Mitragynine from Kratom Leaves (an Alternative Recreational Drug) with Efficient Extraction Method

BSC-Ursa Minor 122

Mitragyna speciosa, also known as Kratom, is an evergreen tree native to Southeast Asia that has been used to relieve pain. The leaves can be chewed, processed into capsular tablet, made into tea, and occasionally smoked. The primary active alkaloid, mitragynine, found in Kratom affects the same receptor as opioids such as heroine. The use of Kratom as an alternative recreational drug has been increasing. Depending on the dosage, consumption of Kratom can lead to psychotic symptoms and various effects. Consequently, the potential effects of Kratom on humans draw international concerns. The purpose of this study is to develop an efficient extraction method for mitragynine from dried Kratom. Grounded Kratom leaves were soaked in methanol and sonicated with a mixture of methanol and chloroform to yield Kratom extract that was rich in alkaloid. The pH of the Kratom extract was adjusted to 9.5 by sodium carbonate solution. The alkaloids were subsequently extracted into chloroform from the basified solution and the resulting dried residue was re-dissolved in 4.0 mL of methanol and then filtered through a 0.45 µm syringe filter prior to injection to reversed-phase HPLC with a C-18 column. The sample was eluted isocratically with a mobile phase of 0.05% formic acid and acetonitrile at pH 5.0 (50:50, v/v), flow rate = 1.0 mL/min. Mitragynine was detected at 223 nm with a retention time of 2.0 min. A calibration curve was prepared with mitragynine standards. Linear correlation was excellent (R2 ­=0.9987). The amount of mitragynine in Kratom was determined (mg/g).