Presentation Title

The Importance of Virulence Factors in the Growth and Infection Cycle of Listeria

Faculty Mentor

Kurt Schesser

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 42

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Listeria monocytogenes is a common bacterium that causes human infections, like miscarriage and septicemia. Listeria uses specific virulence factors to produce proteins that will assist in invasion, replication, and escape. By manipulation of the virulence factors through knockout mutants, this study observed their role and importance in the infection and proliferation life cycle. JEG-3 cells, a human placental line, were infected with wild type Listeria or knockout mutants of individual virulence factors, Internalin A&B, Listeriolysin O, and ActA. Through Colony Forming Unit Assay, it was possible to analyze the number of colonies representing the number of Listeria bacteria after definitive time points. Each virulence factor did play a significant role in the growth and infection of Listeria in the JEG-3 cells as fewer colonies were found in the knockout mutant plates than the wild type. Each virulence factor affected a distinct portion of the invasion, replication, and escape cycle. The omission of a single virulence factor will significantly disrupt the growth and infection life cycle of Listeria. The role of the Listerial virulence factors were tested in JEG-3 cells, but can have different roles in different cell types, like macrophages. Further studies must be done to determine the effect of these specific virulence factors in other cell lines.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

The Importance of Virulence Factors in the Growth and Infection Cycle of Listeria

BSC-Ursa Minor 42

Listeria monocytogenes is a common bacterium that causes human infections, like miscarriage and septicemia. Listeria uses specific virulence factors to produce proteins that will assist in invasion, replication, and escape. By manipulation of the virulence factors through knockout mutants, this study observed their role and importance in the infection and proliferation life cycle. JEG-3 cells, a human placental line, were infected with wild type Listeria or knockout mutants of individual virulence factors, Internalin A&B, Listeriolysin O, and ActA. Through Colony Forming Unit Assay, it was possible to analyze the number of colonies representing the number of Listeria bacteria after definitive time points. Each virulence factor did play a significant role in the growth and infection of Listeria in the JEG-3 cells as fewer colonies were found in the knockout mutant plates than the wild type. Each virulence factor affected a distinct portion of the invasion, replication, and escape cycle. The omission of a single virulence factor will significantly disrupt the growth and infection life cycle of Listeria. The role of the Listerial virulence factors were tested in JEG-3 cells, but can have different roles in different cell types, like macrophages. Further studies must be done to determine the effect of these specific virulence factors in other cell lines.