Presentation Title

The Use of Pruning, Cytokinin Application, and Fertilizer Application to Increase Flush in Murraya koenigii ((L.) (Rutaceae)) and Subsequent Egg Laying by ACP (Diaphorina citri, Kuwayama) (Hemiptera: Psyllidae)

Faculty Mentor

Anna Soper

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 58

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Tamarixia radiata (Waterson) (Hymenoptera: Eulophidae) has been identified as a biological control agent of the Asian Citrus Psyllid (ACP), Diaphorina citri, Kuwayama (Hemiptera: Psyllidae). This psyllid has been shown to vector Huanglongbing (HLB), which is a disease that has crippled the Florida citrus industry. Efforts by the California Department of Food and Agriculture (CDFA) have been made to rear and release T. radiata to control the spread of D. citri before the psyllid reaches dangerous population levels in California. In order to adequately study the psyllid and experiment methods to eliminate the spread of the disease, a substantial population of ACP in a controlled environment is essential. A host plant for the rearing of D. citri to feed reared populations of T. radiata is necessary, in which case Murraya koenigii (L.) (Rutaceae), or the Curry Leaf plant, is used. The Curry Leaf plant can support populations of ACP, but only if the plant continuously produces new, green growth which the ACP require to breed and feed on. Different regiments of pruning, Cytokinin application, and fertilizer application were tested to evaluate their efficacy in inducing flush in previously pruned M. koenigii plants. Thereafter, ACP were placed on the plants to observe which regiment produced the most appealing flush for reproduction of the psyllid.

Summary of research results to be presented

By comparing the flush growth one and two weeks after treatment, it was shown that fertilizer application alone is not enough to cause sustained flush production. Although there were leaders in total flush production, by the seventh week of the experiment there was no significant difference in the flush produced between the groups. However, certain groups flushed very quickly after treatment meaning that these plants could be used for the rearing of ACP very quickly after treatment.

In future experiments, the use of different plant hormones and the investigation of different forms of cytokinin is recommended. Efforts to isolate the effects of the hormones should also be examined.

Although there was a group that was the leader in observed ACP egg-load, it was not the leader in flush growth. This further emphasizes that the goal is to produce quality flush for the ACP to feed and lay on, not necessarily large quantities of flush.

Many ACP eggs were also found on the mature leaflets of the M. koenigii as opposed to the usual flush or younger leaves. This could indicate that the adult ACP wanted to oviposit eggs, but could not find a more suitable location.

In further experiments, flush production and egg-laying should be examined continuously. Treated M. koenigii should be inoculated with ACP at most 3 weeks after treatment to ensure the quality of flush and to increase the number of replications for the egg-laying component. No-choice tests should also be utilized.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

The Use of Pruning, Cytokinin Application, and Fertilizer Application to Increase Flush in Murraya koenigii ((L.) (Rutaceae)) and Subsequent Egg Laying by ACP (Diaphorina citri, Kuwayama) (Hemiptera: Psyllidae)

BSC-Ursa Minor 58

Tamarixia radiata (Waterson) (Hymenoptera: Eulophidae) has been identified as a biological control agent of the Asian Citrus Psyllid (ACP), Diaphorina citri, Kuwayama (Hemiptera: Psyllidae). This psyllid has been shown to vector Huanglongbing (HLB), which is a disease that has crippled the Florida citrus industry. Efforts by the California Department of Food and Agriculture (CDFA) have been made to rear and release T. radiata to control the spread of D. citri before the psyllid reaches dangerous population levels in California. In order to adequately study the psyllid and experiment methods to eliminate the spread of the disease, a substantial population of ACP in a controlled environment is essential. A host plant for the rearing of D. citri to feed reared populations of T. radiata is necessary, in which case Murraya koenigii (L.) (Rutaceae), or the Curry Leaf plant, is used. The Curry Leaf plant can support populations of ACP, but only if the plant continuously produces new, green growth which the ACP require to breed and feed on. Different regiments of pruning, Cytokinin application, and fertilizer application were tested to evaluate their efficacy in inducing flush in previously pruned M. koenigii plants. Thereafter, ACP were placed on the plants to observe which regiment produced the most appealing flush for reproduction of the psyllid.