Presentation Title

Asymmetric Cobalticenium Molecules for Mediated Electrochemical Biocatalysis

Faculty Mentor

Andrew K. Udit

Start Date

18-11-2017 9:59 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 145

Session

Poster 1

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Cytochrome P450 is a class of hemeproteins found in nearly all known life. This enzyme is critical to detoxification and bioactivation of most known pharmaceuticals. Its use in industry could lead to the ability to manufacture powerful new drug derivatives. Moreover, P450 has the potential to create a safer, more economic method for the testing of new drugs. Current laboratory uses of the enzyme are inefficient and require the use of an expensive mediator molecule called NAD(P)H. NAD(P)H serves as a source of electrons to reduce P450 so that it may function, but cost prohibits its use in many research laboratories. In contrast, cobaltocenium molecules that have the potential to act in place of NAD(P)H can be synthesized cheaply and in large quantities. This research project investigates the synthesis and use of an asymmetric, bifunctional cobaltocenium molecule to serve as an electron relay between an electrode and the enzyme, thus eliminating the need for NAD(P)H. We will employ a variety of spectroscopic techniques, such as 1H-NMR and mass spectroscopy, as well as electrochemical methods to characterize the molecule. Once confirmed, we will begin testing its redox characteristics, as well as its potential as a mediator in P450 biocatalysis.

Summary of research results to be presented

Currently, we have managed to synthesize an asymmetric, bifunctionalized cobaltocenium derivative suitable for electrochemical biocatalysis. NMR integration data supports the structure we suggest; however, because of the similar electronic characteristics between our functional groups, as well as the strong effect of the cobalt on hydrogen nuclei, we were unable to differentiate the predicted peaks from the NMR spectrum. We are in the process of performing electrochemical measurements to confirm the presence of necessary functional groups on the cobaltocenium moiety. In addition to this, mass spectroscopy has been used to confirm the molecular weight. Once we have verified our results electrochemically, we will proceed to attaching our molecule to the protein and an electrode before testing the redox ability of the system.

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Nov 18th, 9:59 AM Nov 18th, 11:00 AM

Asymmetric Cobalticenium Molecules for Mediated Electrochemical Biocatalysis

BSC-Ursa Minor 145

Cytochrome P450 is a class of hemeproteins found in nearly all known life. This enzyme is critical to detoxification and bioactivation of most known pharmaceuticals. Its use in industry could lead to the ability to manufacture powerful new drug derivatives. Moreover, P450 has the potential to create a safer, more economic method for the testing of new drugs. Current laboratory uses of the enzyme are inefficient and require the use of an expensive mediator molecule called NAD(P)H. NAD(P)H serves as a source of electrons to reduce P450 so that it may function, but cost prohibits its use in many research laboratories. In contrast, cobaltocenium molecules that have the potential to act in place of NAD(P)H can be synthesized cheaply and in large quantities. This research project investigates the synthesis and use of an asymmetric, bifunctional cobaltocenium molecule to serve as an electron relay between an electrode and the enzyme, thus eliminating the need for NAD(P)H. We will employ a variety of spectroscopic techniques, such as 1H-NMR and mass spectroscopy, as well as electrochemical methods to characterize the molecule. Once confirmed, we will begin testing its redox characteristics, as well as its potential as a mediator in P450 biocatalysis.