Presentation Title

BPM Plot: A Software for Standardization of Post-Acquisition Nanoparticle Tracking Analysis with ZetaVIEW® (Particle-Metrix)

Faculty Mentor

Massimo S. Fiandaca, MD/MBA, Howard J. Federoff, MD/PhD

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 86

Session

Poster 1

Type of Presentation

Poster

Subject Area

engineering_computer_science

Abstract

Nanoparticle tracking analytics (NTA) (e.g. ZetaVIEW®) have emerged as powerful platforms for the characterization and quantification of extracellular vesicle (EV) populations. NTA provides a robust, semi-automated method for quantifying the average particle number and size range within a standardized volume. Despite improved reproducibility and validity of NTA, as confirmed by electron microscopy (EM), the EV-field still lacks an efficient workflow for the post-acquisition phase of experimental analysis and standardized presentation of results in publications. Regarding the latter, transparency relating to pre/post-acquisition parameters, such as brightness, min/max pixel size, shutter speed, and frame rate settings, and variability between replicate samples, remain major hurdles to reproducibility. We developed a custom MATLAB® application (BPM Plot) in an attempt to improve ZetaVIEW® NTA reproducibility. Our software platform allows the user to load multiple sample replicates via ZetaVIEW® .txt file acquisition measurements from a designated folder path directory. Once loaded, the queued files are run through an efficient algorithm that displays publication-ready bar plots of the mean vesicle diameter and concentration and size distribution figures across all replicates. To improve upon transparency, our application displays standard error bars for all figures, along with the option to filter and smooth the raw data using multiple moving average algorithms. Lastly, our application queues all loaded files to ensure each have the same acquisition parameters; with a warning displayed if the parameters are not the same across samples. BPM Plot is an adjunct software component for NTA to optimize the post-acquisition data processing and presentation.

Keywords: ZetaVIEW®; MATLAB®; Nanoparticle Tracking Analytics (NTA); EVs; Exosomes

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

BPM Plot: A Software for Standardization of Post-Acquisition Nanoparticle Tracking Analysis with ZetaVIEW® (Particle-Metrix)

BSC-Ursa Minor 86

Nanoparticle tracking analytics (NTA) (e.g. ZetaVIEW®) have emerged as powerful platforms for the characterization and quantification of extracellular vesicle (EV) populations. NTA provides a robust, semi-automated method for quantifying the average particle number and size range within a standardized volume. Despite improved reproducibility and validity of NTA, as confirmed by electron microscopy (EM), the EV-field still lacks an efficient workflow for the post-acquisition phase of experimental analysis and standardized presentation of results in publications. Regarding the latter, transparency relating to pre/post-acquisition parameters, such as brightness, min/max pixel size, shutter speed, and frame rate settings, and variability between replicate samples, remain major hurdles to reproducibility. We developed a custom MATLAB® application (BPM Plot) in an attempt to improve ZetaVIEW® NTA reproducibility. Our software platform allows the user to load multiple sample replicates via ZetaVIEW® .txt file acquisition measurements from a designated folder path directory. Once loaded, the queued files are run through an efficient algorithm that displays publication-ready bar plots of the mean vesicle diameter and concentration and size distribution figures across all replicates. To improve upon transparency, our application displays standard error bars for all figures, along with the option to filter and smooth the raw data using multiple moving average algorithms. Lastly, our application queues all loaded files to ensure each have the same acquisition parameters; with a warning displayed if the parameters are not the same across samples. BPM Plot is an adjunct software component for NTA to optimize the post-acquisition data processing and presentation.

Keywords: ZetaVIEW®; MATLAB®; Nanoparticle Tracking Analytics (NTA); EVs; Exosomes