Presentation Title

Carbapenem-resistant (CR) Ochrobactrum anthropi: Characterization of the Strains Involved in an Outbreak

Faculty Mentor

Maria Soledad Ramirez

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 37

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

In 2016 an outbreak caused by O. anthropi occurred in a hospital. Surprisingly the four isolates involved in the outbreak were carbapenem-resistance. The aim of this project was to characterize the strains involved in the outbreak and identify the resistant determinants that can explain the multidrug-resistance (MDR) phenotype observed. Four isolates were recovered from the blood sample of four patients. The isolates were identified as O. anthropi and susceptibility tests were performed. PCR reactions were carried out and OD-PCR was used to determine their genetic relationship. In addition, the draft genome sequence of one of the strains (OA 107383) was obtained.

All isolates were MDR and EDTA synergies exhibited positive results, suggesting the presence of a MBL in each isolate. However, PCR reaction for blaIMP, blaVIM, blaKPC and blaNDM were negative. The same pattern of bands was obtained by OD-PCR suggesting an epidemiological relationship. The whole genome sequencing of OA 107383 was performed and its draft genome consists of 4,792,719-bp sequences. By RAST server, 4554 protein-coding genes were predicted. Sequence analysis of the genome identified the presence of multiple resistance genes, and most importantly, a metal-depended hydrolase of the β -lactamase superfamily I, phnP. Moreover, genes coding for efflux pumps were identified and two intact phages were also found in OA 107383 genome.

This is the first description of an outbreak cause by CR O. anthropi. We have identified different resistance genes in the genome of O. anthropi. The presence of these determinants could explain the observed resistance phenotype.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

Carbapenem-resistant (CR) Ochrobactrum anthropi: Characterization of the Strains Involved in an Outbreak

BSC-Ursa Minor 37

In 2016 an outbreak caused by O. anthropi occurred in a hospital. Surprisingly the four isolates involved in the outbreak were carbapenem-resistance. The aim of this project was to characterize the strains involved in the outbreak and identify the resistant determinants that can explain the multidrug-resistance (MDR) phenotype observed. Four isolates were recovered from the blood sample of four patients. The isolates were identified as O. anthropi and susceptibility tests were performed. PCR reactions were carried out and OD-PCR was used to determine their genetic relationship. In addition, the draft genome sequence of one of the strains (OA 107383) was obtained.

All isolates were MDR and EDTA synergies exhibited positive results, suggesting the presence of a MBL in each isolate. However, PCR reaction for blaIMP, blaVIM, blaKPC and blaNDM were negative. The same pattern of bands was obtained by OD-PCR suggesting an epidemiological relationship. The whole genome sequencing of OA 107383 was performed and its draft genome consists of 4,792,719-bp sequences. By RAST server, 4554 protein-coding genes were predicted. Sequence analysis of the genome identified the presence of multiple resistance genes, and most importantly, a metal-depended hydrolase of the β -lactamase superfamily I, phnP. Moreover, genes coding for efflux pumps were identified and two intact phages were also found in OA 107383 genome.

This is the first description of an outbreak cause by CR O. anthropi. We have identified different resistance genes in the genome of O. anthropi. The presence of these determinants could explain the observed resistance phenotype.